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Fluorescent Microscope Slides and Alignment Targets![]()
FSK5 Set of All Five Colored Slides ADF1 Blue Alignment Disk with Target ADF5 Red Alignment Disk with Target ADF7 Green Alignment Disk with Hole in Disk Center FSK3 Yellow Slide CPA4 Orange 30 mm Cage Alignment Plate ![]() Please Wait ![]() Click to Enlarge The FSK6 Red Fluorescent Slide Mounted Using the MP100-RCH2 Rigid Stand Slide Holder Features
Thorlabs offers a selection of Fluorescent Microscope Slides and Alignment Targets for use with widefield, confocal, or multiphoton fluorescence imaging systems. Manufactured from a cast acrylic substrate, they are available in five different colors: blue, green, yellow, orange, and red. Each color has a fluorescence spectrum similar to a variety of fluorophores; see the Fluorescence Graphs tab for more details. These slides, disks, and plates provide a continuous fluorescent field so that field of view, illumination consistency, and sample alignment may be assessed and adjusted prior to using a sample containing a fluorophore. The fluorescent microscope slides are compatible with our sample holders that fit 26 mm x 76 mm, 1.7 ± 0.5 mm thick slides. Each slide is available individually, in packs of five, or in a slide set that includes one slide of each color. The fluorescent alignment disks can be mounted using our Ø1" fixed optic mounts, lens tubes, or 30 mm cage plates. Each disk is available individually or in packs of five. The fluorescent cage alignment plates are engraved with concentric circles and have a central through hole aligned with the center of Thorlabs' cage systems. These drop in alignment tools are available individually for 16 mm, 30 mm, and 60 mm cage systems. For custom color, sizes, and shapes up to 1' x 2', please contact Tech Support. These data are typical; performance may vary from lot to lot. ![]() Click to Enlarge Raw Data Measured fluorescence spectrum of the FSK1 blue fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. The ADF1 and ADF6 blue fluorescent disks share the same fluorescence spectrum as the FSK1 blue fluorescent slide. ![]() Click to Enlarge Raw Data Measured fluorescence spectrum of the FSK2 green fluorescent slide when illuminated with the M470F3 470 nm Fiber-Coupled LED. The ADF2 and ADF7 green fluorescent disks share the same fluorescence spectrum as the FSK2 green fluorescent slide. ![]() Click to Enlarge Raw Data Measured fluorescence spectrum of the FSK3 yellow fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. The ADF3 and ADF8 yellow fluorescent disks share the same fluorescence spectrum as the FSK3 yellow fluorescent slide. ![]() Click to Enlarge Raw Data Measured fluorescence spectrum of the FSK4 orange fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. The ADF4 and ADF9 orange fluorescent disks share the same fluorescence spectrum as the FSK4 orange fluorescent slide. ![]() Click to Enlarge Raw Data Measured fluorescence spectrum of the FSK6 red fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. The ADF5 and ADF10 red fluorescent disks share the same fluorescence spectrum as the FSK6 red fluorescent slide. This data is typical; performance may vary from lot to lot. ![]() Click to Enlarge Raw Data Comparison of transmission measurements of the FSK1 (blue), FSK2 (green), FSK3 (yellow), FSK4 (orange), and FSK6 (red) fluorescent slides from 300 nm to 700 nm. The ADF1/ADF6 (blue), ADF2/ADF7 (green), ADF3/ADF8 (yellow), ADF4/ADF9 (orange), and ADF5/ADF10 (red) fluorescent disks and the SCPA2, CPA3, CPA4, and LCPA2 orange fluorescent plates share the same transmission as the fluorescent slide with the identical color. ![]() Click to Enlarge Transmission measurements of two FSK1 blue fluorescent slides. The ADF1 and ADF6 blue fluorescent disks share the same transmission as the FSK1 blue fluorescent slide. ![]() Click to Enlarge Transmission measurements of two FSK2 green fluorescent slides. The ADF2 and ADF7 green fluorescent disks share the same transmission as the FSK2 green fluorescent slide. ![]() Click to Enlarge Transmission measurements of two FSK3 yellow fluorescent slides. The ADF3 and ADF8 yellow fluorescent disks share the same transmission as the FSK3 yellow fluorescent slide. ![]() Click to Enlarge Transmission measurements of two FSK4 orange fluorescent slides. The ADF4 and ADF9 orange fluorescent disks and the SCPA2, CPA3, CPA4, and LCPA2 orange fluorescent plates share the same transmission as the FSK4 orange fluorescent slide. ![]() Click to Enlarge Transmission measurements of two FSK6 red fluorescent slides. The ADF5 and ADF10 red fluorescent disks share the same transmission as the FSK6 red fluorescent slide.
![]() ![]() Click to Enlarge The FSK5 Slide Set and slides sold in packs of five come in a box for safe storage.
Our Fluorescent Microscope Slides are designed to be used to assess and align sample placement and illumination levels in fluorescence imaging systems. Each slide is 25.4 mm x 76.2 mm and is cut from cast acrylic with a thickness of 1.7 ± 0.5 mm. A plastic storage box is included with the FSK5 Slide Set and the packs of five slides to keep them clean and dust-free. The box is engraved with the Thorlabs logo and item # on the top, as shown in the image to the right. For custom sizes up to 1' x 2', please contact Tech Support. ![]() ![]() Click to Enlarge The ADF5 Red Fluorescent Disk Mounted in an LMR1 Mount
Our Fluorescent Alignment Disks with Target Guides are designed to aid in the alignment of fluorescence imaging systems. Each disk is 1" in diameter and is cut from cast acrylic material with a thickness of 1.7 ± 0.5 mm. A target with two concentric circles is engraved on every disk. For custom sizes up to 1' x 2', please contact Tech Support. ![]() ![]() Click to Enlarge ADF9 Orange Fluorescent Disk Mounted in a CP33T Cage Plate
Our Fluorescent Alignment Disks with Holes are designed to aid in the alignment of fluorescence imaging systems. Each disk is 1" in diameter and is cut from cast acrylic material with a thickness of 1.7 ± 0.5 mm. A Ø1.5 mm hole is cut into the center of every disk. For custom sizes up to 1' x 2', please contact Tech Support. ![]()
These drop-in alignment plates are convenient tools for spatial calibration and alignment of cage-based fluorescence imaging systems. The cast acrylic substrate produces fluorescence spectra similar to orange fluorescing proteins and dyes. These plates provide a continuous fluorescent field so that the field of view, illumination consistency, and sample alignment may be assessed and adjusted prior to using a sample containing a fluorophore. | |||||||||||||||||
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