Products Home / Optical Elements / Test Targets, Calibration Targets, and Reticles / Fluorescent Microscope Slides and Alignment TargetsFluorescent Microscope Slides and Alignment Targets
- 25.4 mm x 76.2 mm Fluorescent Slides, and Ø1" Fluorescent Disks
- Cage Alignment Plates for 16 mm, 30 mm, and 60 mm Cage Systems
- Available in Five Colors: Blue, Green, Yellow, Orange, and Red
- Made of Cast Acrylic Substrate
- Provides a Uniform Fluorescent Field Across the Surface Area
FSK5
Set of All Five Colored Slides
ADF1
Blue Alignment Disk with Target
ADF5
Red Alignment Disk with Target
ADF7
Green Alignment Disk with Hole in Disk Center
FSK3
Yellow Slide
CPA4
Orange 30 mm Cage Alignment Plate
Please Wait
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The FSK6 Red Fluorescent Slide Mounted Using the MP100-RCH2 Rigid Stand Slide Holder
Features
- Assess and Align Fluorescence Imaging Systems
- Five Different Fluorescence Spectra Similar to a Variety of Fluorophores (See the Fluorescence Graphs Tab)
- 25.4 mm x 76.2 mm, 1.7 mm Thick Fluorescent Slides
- Ø1", 1.7 mm Thick Fluorescent Disks with Engraved Target or Hole in Disk Center
- 1.7 mm Thick Orange Fluorescent Cage Alignment Plates for 16 mm, 30 mm, and 60 mm Cage Systems
Thorlabs offers a selection of Fluorescent Microscope Slides and Alignment Targets for use with widefield, confocal, or multiphoton fluorescence imaging systems. Manufactured from a cast acrylic substrate, they are available in five different colors: blue, green, yellow, orange, and red. Each color has a fluorescence spectrum similar to a variety of fluorophores; see the Fluorescence Graphs tab for more details. These slides, disks, and plates provide a continuous fluorescent field so that field of view, illumination consistency, and sample alignment may be assessed and adjusted prior to using a sample containing a fluorophore.
The fluorescent microscope slides are compatible with our sample holders that fit 26 mm x 76 mm, 1.7 ± 0.5 mm thick slides. Each slide is available individually, in packs of five, or in a slide set that includes one slide of each color. The fluorescent alignment disks can be mounted using our Ø1" fixed optic mounts, lens tubes, or 30 mm cage plates. Each disk is available individually or in packs of five. The fluorescent cage alignment plates are engraved with concentric circles and have a central through hole aligned with the center of Thorlabs' cage systems. These drop in alignment tools are available individually for 16 mm, 30 mm, and 60 mm cage systems.
For custom color, sizes, and shapes up to 1' x 2', please contact Tech Support.
These data are typical; performance may vary from lot to lot.
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Raw Data
Measured fluorescence spectrum of the FSK1 blue fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. The ADF1 and ADF6 blue fluorescent disks share the same fluorescence spectrum as the FSK1 blue fluorescent slide.
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Raw Data
Measured fluorescence spectrum of the FSK2 green fluorescent slide when illuminated with the M470F3 470 nm Fiber-Coupled LED. The ADF2 and ADF7 green fluorescent disks share the same fluorescence spectrum as the FSK2 green fluorescent slide.
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Raw Data
Measured fluorescence spectrum of the FSK3 yellow fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. The ADF3 and ADF8 yellow fluorescent disks share the same fluorescence spectrum as the FSK3 yellow fluorescent slide.
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Raw Data
Measured fluorescence spectrum of the FSK4 orange fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. The ADF4 and ADF9 orange fluorescent disks share the same fluorescence spectrum as the FSK4 orange fluorescent slide.
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Raw Data
Measured fluorescence spectrum of the FSK6 red fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. The ADF5 and ADF10 red fluorescent disks share the same fluorescence spectrum as the FSK6 red fluorescent slide.
This data is typical; performance may vary from lot to lot.
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Raw Data
Comparison of transmission measurements of the FSK1 (blue), FSK2 (green), FSK3 (yellow), FSK4 (orange), and FSK6 (red) fluorescent slides from 300 nm to 700 nm. The ADF1/ADF6 (blue), ADF2/ADF7 (green), ADF3/ADF8 (yellow), ADF4/ADF9 (orange), and ADF5/ADF10 (red) fluorescent disks and the SCPA2, CPA3, CPA4, and LCPA2 orange fluorescent plates share the same transmission as the fluorescent slide with the identical color.
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Transmission measurements of two FSK1 blue fluorescent slides. The ADF1 and ADF6 blue fluorescent disks share the same transmission as the FSK1 blue fluorescent slide.
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Transmission measurements of two FSK2 green fluorescent slides. The ADF2 and ADF7 green fluorescent disks share the same transmission as the FSK2 green fluorescent slide.
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Transmission measurements of two FSK3 yellow fluorescent slides. The ADF3 and ADF8 yellow fluorescent disks share the same transmission as the FSK3 yellow fluorescent slide.
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Transmission measurements of two FSK4 orange fluorescent slides. The ADF4 and ADF9 orange fluorescent disks and the SCPA2, CPA3, CPA4, and LCPA2 orange fluorescent plates share the same transmission as the FSK4 orange fluorescent slide.
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Transmission measurements of two FSK6 red fluorescent slides. The ADF5 and ADF10 red fluorescent disks share the same transmission as the FSK6 red fluorescent slide.
| Posted Comments: | |
Lothar Schmid
(posted 2021-06-30 09:50:51.8) Hi Thorlabs team,
I have a few questions regarding the fluorescent microscope slides, which I would like to use for intensity calibration of a fluorescent reader:
* How reproducible is the fluorescence intensity from batch to batch?
* Do the spectra change over time?
* Do the slides show bleaching of fluorophores?
* Is the fluorescence temperature-dependent, for a temperature range between 0 and 60 degC?
Thanks,
Lothar Schmid cdolbashian
(posted 2021-07-20 08:28:30.0) Thank you for your interest in Thorlabs products. These materials are, unfortunately, not characterized in this manner. All data we have for them is provided on the web page. I have reached out to your to discuss your application directly. Gary Riggott
(posted 2021-02-17 12:53:43.627) Hi-
The results I've gotten with my fluorimeter do not match your reference spectra very well and I'm wondering if you have any thoughts on this.
Blue Std:
My result shows a broad peak from about 400 to 480nm that doesn't exist in the spectrum for the standard.
Green Std:
I get a broad peak from 480 to about 560nm with a max at 501. The reference shows a well-defined big peak at 499nm.
Orange Std:
I get a broad peak from about 580 to 610 with a max at 587. The reference shows a minor peak at 612, and I don't have anything there.
Yellow Std:
My only peak is at 366nm. The reference shows a major peak at 493 and a minor peak at 517. This is the worst agreement between measured and expected among any of the standards.
Red Std:
I get a peak at 566 and a broad peak from about 580-630, with a max at 598. The reference shows peaks at 472, a big one at 586 and a broad peak from 620-625. I have no peaks near 472nm.
I welcome any input.
If you could add a second "Run" or spectrum as you did with the %Transmission curves, that would be a huge help!
Thank you,
Gary YLohia
(posted 2021-04-14 02:36:20.0) Hello Gary, thank you for contacting Thorlabs. After going over the data you sent us and talking to our team here, it seems like the discrepancy that you are seeing ranges from 3 - 10 nm, which can be attributed to a lot-to-lot variation. These are not intended for use as a calibration spectra but are rather for field of view, illumination consistency, and sample alignment assessment. Vincent Khau
(posted 2020-12-01 18:18:29.887) Do you have data on the fluorescence variation from batch to batch for the blue fluorescence slide? Is there a certain tolerance that these slides are manfuactured to in terms of their fluorescence response?
Thank you. YLohia
(posted 2020-12-15 02:49:19.0) Hello, thank you for contacting Thorlabs. Unfortunately, we do not batch-test these and we do not control for a specific tolerance of the fluorescence response of these slides. Dmitry Gorbachev
(posted 2020-02-21 03:38:27.007) Hello! I work with Frequency-Domain FLIM, and I used your fluorescent slides kit FSK5 to tune our FLIM camera. I want to know if it is possible to order the same set of slides from you, but larger? for example 10cm in diameter. Thanks a lot! llamb
(posted 2020-02-25 02:12:46.0) Thank you for contacting Thorlabs. Yes, custom fluorescence slides are indeed feasible. We will reach out to you directly to quote this. Nikola Krstajic
(posted 2019-07-12 09:39:00.04) I work in near-infrared and fluorescence targets geared for ICG exc/emission (785nm exc) would really help me and all colleagues working in this are. I am 100% sure you would sell many. It is also not clear if your fluorescence targets work on 625nm excitation as your spectra show wavelengths below exc and this is really unusual (antistokes is an exception in fluorescence). Thanks a lot! YLohia
(posted 2019-07-23 09:46:29.0) Hello, thank you for your feedback. I have posted your request on our internal engineering forum for further consideration as a future product. petur.hermannsson
(posted 2019-01-21 10:33:44.437) Do you have numbers on the batch-to-batch consistency/uniformity of these slides, as well as the rate of photobleaching? YLohia
(posted 2019-01-28 11:03:57.0) Hello, thank you for contacting Thorlabs. We have actually just updated our web presentation to include some information on the consistency between two runs, and this information can be found in the "Transmission Graphs" tab. That being said, unfortunately, we do not have information on the photobleaching at the moment. |
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The FSK5 Slide Set and slides sold in packs of five come in a box for safe storage.
- Fluorescent Slides Available in Five Colors: Blue, Green, Yellow, Orange, and Red
- Each Slide Sold Individually or in Packs of Five
- FSK5 Slide Set Includes All Five Colored Slides
- 25.4 mm x 76.2 mm and 1.7 mm Thick
- Compatible with Our Sample Holders for 26 mm x 76 mm, 1.7 mm ± 0.5 mm Thick Slides
- Engraved Storage Box Included with FSK5 Slide Set and Packs of Five
Our Fluorescent Microscope Slides are designed to be used to assess and align sample placement and illumination levels in fluorescence imaging systems. Each slide is 25.4 mm x 76.2 mm and is cut from cast acrylic with a thickness of 1.7 ± 0.5 mm. A plastic storage box is included with the FSK5 Slide Set and the packs of five slides to keep them clean and dust-free. The box is engraved with the Thorlabs logo and item # on the top, as shown in the image to the right. For custom sizes up to 1' x 2', please contact Tech Support.
These slides are compatible with our sample holders that fit 26 mm x 76 mm slides ≥1.7 mm thick. Please note that our C4SH01 Four-Position Microscope Slide Holder is not compatible with these slides.
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The ADF5 Red Fluorescent Disk Mounted in an LMR1 Mount
- Fluorescent Disks Available in Five Colors: Blue, Green, Yellow, Orange, and Red
- Each Disk Sold Individually or in Packs of Five
- Ø1" and 1.7 mm Thick
- Engraved with Target Guide Lines, and Ø3.4 mm and Ø9.4 mm Concentric Circles
- Compatible with Our Ø1" Fixed Optic Mounts, Lens Tubes, or 30 mm Cage Plates
Our Fluorescent Alignment Disks with Target Guides are designed to aid in the alignment of fluorescence imaging systems. Each disk is 1" in diameter and is cut from cast acrylic material with a thickness of 1.7 ± 0.5 mm. A target with two concentric circles is engraved on every disk. For custom sizes up to 1' x 2', please contact Tech Support.
These disks can be mounted with our Ø1" fixed optic mounts, lens tubes, or 30 mm cage plates, as shown in the image to the right.
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ADF9 Orange Fluorescent Disk Mounted in a CP33T Cage Plate
- Fluorescent Disks Available in Five Colors: Blue, Green, Yellow, Orange, and Red
- Each Disk Sold Individually or in Packs of Five
- Ø1" and 1.7 mm Thick
- Ø1.5 ± 0.15 mm Holes in Disk Centers for Alignment
- Compatible with Our Ø1" Fixed Optic Mounts, Lens Tubes, or 30 mm Cage Plates
Our Fluorescent Alignment Disks with Holes are designed to aid in the alignment of fluorescence imaging systems. Each disk is 1" in diameter and is cut from cast acrylic material with a thickness of 1.7 ± 0.5 mm. A Ø1.5 mm hole is cut into the center of every disk. For custom sizes up to 1' x 2', please contact Tech Support.
These disks can be mounted with our Ø1" fixed optic mounts, lens tubes, or 30 mm cage plates as shown in the image to the right.
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- Orange Fluorescing Cast Acrylic Plates for 16 mm, 30 mm, and 60 mm Cage Systems
- Guide Lines and Ø1 mm or Ø5 mm Through Hole Aligned at Center of Compatible Cage System
- Quick, Drop-In Alignment Tools for Fluorescence Imaging Systems
These drop-in alignment plates are convenient tools for spatial calibration and alignment of cage-based fluorescence imaging systems. The cast acrylic substrate produces fluorescence spectra similar to orange fluorescing proteins and dyes. These plates provide a continuous fluorescent field so that the field of view, illumination consistency, and sample alignment may be assessed and adjusted prior to using a sample containing a fluorophore.
Each plate is cut from cast acrylic material with a thickness of 1.7 mm and has either a Ø1 mm or Ø5 mm through hole aligned at the center of the cage assembly. Each through hole is surrounded by concentric, engraved alignment rings. Options are available for compatibility with our 16 mm, 30 mm, and 60 mm cage systems. For custom colors and sizes up to 1' x 2', please contact Tech Support.
| Item # | Color | Through Hole Diameter | Alignment Ring Diameters | Compatible Cage System | Thickness | Transmission (Click for Graph) |
Requires Charging |
|---|---|---|---|---|---|---|---|
| SCPA2 | Orange | 1 mm (0.04") | 3 mm, 5 mm, 7 mm, and 9 mm | 16 mm | 1.7 mm ± 0.5 mm |  |
No |
| CPA3 | 1 mm (0.04") | 4 mm, 7 mm, 10 mm, and 13 mm | 30 mm | ||||
| CPA4 | 5 mm (0.20") | 7 mm, 10 mm, and 13 mm | 30 mm | ||||
| LCPA2 | 1 mm (0.04") | 7 mm, 12 mm, 17 mm, 22 mm, and 27 mm | 60 mm |
 Fluorescent Slides and Disks |