Create an Account  |   Log In

View All »Matching Part Numbers


Your Shopping Cart is Empty
         

Fluorescent Calibration Slides and Alignment Disks


  • 25.4 mm x 76.2 mm Fluorescent Slides and Ø1" Fluorescent Disks
  • Available in Five Colors: Blue, Green, Yellow, Orange, and Red 
  • Made of Cast Acrylic Substrate
  • Provides a Uniform Fluorescent Field Across the Surface Area

FSK5

Set of All Five Colored Slides

ADF1

Blue Alignment Disk

ADF6

Red Alignment Disk

ADF2

Green Alignment Disk

FSK3

Yellow Calibration Slide

FSK4

Orange Calibration Slide

Related Items


Please Wait

Click to Enlarge
The FSK6 Red Fluorescent Slide Mounted Using the MP100-RCH2 Rigid Stand Slide Holder

Features

  • Spatially Calibrate and Align Fluorescence Imaging Systems
  • Five Different Fluorescence Spectra Similar to a Variety of Fluorophores (See the Fluorescence Graphs Tab) 
  • 25.4 mm x 76.2 mm,1.7 mm Thick Fluorescent Slides
  • Ø1", 1.7 mm Thick Fluorescent Disks Engraved with Target for Easy Alignment

Thorlabs offers a selection of Fluorescent Calibration Slides and Alignment Disks designed for fluorescence imaging systems. Manufactured from a cast acrylic substrate, they are each available in five different colors: blue, green, yellow, orange, and red. Each has a fluorescence spectra similar to a variety of fluorophores; see the Fluorescence Graphs tab for more details.

The fluorescent calibration slides are compatible with our sample holders that fit 26 mm x 76 mm slides up to 1.7 mm thick. Each slide is available individually, in packs of five, or in a slide set that includes all five different colored slides. The fluorescent alignment disks can be mounted using our Ø1" fixed optic mounts or lens tubes. Each disk is available individually or in packs of five.

For custom sizes and shapes up to 1' x 2', please contact Tech Support.

This data is typical; performance may vary from lot to lot.


Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK1 blue fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. The ADF1 blue fluorescent disk shares the same fluorescence spectrum as the FSK1 blue fluorescent slide.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK2 green fluorescent slide when illuminated with the M470F3 470 nm Fiber-Coupled LED. The ADF2 green fluorescent disk shares the same fluorescence spectrum as the FSK2 green fluorescent slide.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK3 yellow fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. The ADF3 yellow fluorescent disk shares the same fluorescence spectrum as the FSK3 yellow fluorescent slide.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK4 orange fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. The ADF4 orange fluorescent disk shares the same fluorescence spectrum as the FSK4 orange fluorescent slide.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK6 red fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. The ADF5 red fluorescent disk shares the same fluorescence spectrum as the FSK6 red fluorescent slide.

This data is typical; performance may vary from lot to lot.


Click to Enlarge

Raw Data
Comparison of transmission measurements of the FSK1 (blue), FSK2 (green), FSK3 (yellow), FSK4 (orange), and FSK6 (red) fluorescent slides from 300 nm to 700 nm. The ADF1 (blue), ADF2 (green), ADF3 (yellow), ADF4 (orange), and ADF5 (red) fluorescent disks share the same transmission as the fluorescent slide with the identical color.

Click to Enlarge
Transmission measurements of two FSK1 blue fluorescent slides. The ADF1 blue fluorescent disk shares the same transmission as the FSK1 blue fluorescent slide.

Click to Enlarge
Transmission measurements of two FSK2 green fluorescent slides. The ADF2 green fluorescent disk shares the same transmission as the FSK2 green fluorescent slide.

Click to Enlarge
Transmission measurements of two FSK3 yellow fluorescent slides. The ADF3 yellow fluorescent disk shares the same transmission as the FSK3 yellow fluorescent slide.

Click to Enlarge
Transmission measurements of two FSK4 orange fluorescent slides. The ADF4 orange fluorescent disk shares the same transmission as the FSK4 orange fluorescent slide.

Click to Enlarge
Transmission measurements of two FSK6 red fluorescent slides. The ADF5 red fluorescent disk shares the same transmission as the FSK6 red fluorescent slide.

Posted Comments:
petur.hermannsson  (posted 2019-01-21 10:33:44.437)
Do you have numbers on the batch-to-batch consistency/uniformity of these slides, as well as the rate of photobleaching?
YLohia  (posted 2019-01-28 11:03:57.0)
Hello, thank you for contacting Thorlabs. We have actually just updated our web presentation to include some information on the consistency between two runs, and this information can be found in the "Transmission Graphs" tab. That being said, unfortunately, we do not have information on the photobleaching at the moment.

Fluorescent Calibration Microscope Slides


Click to Enlarge
The FSK5 Slide Set and slides sold in packs of five come in a box for safe storage.
  • Fluorescent Slides Available in Five Colors: Blue, Green, Yellow, Orange, and Red
    • Each Slide Sold Individually or in Packs of Five
    • FSK5 Slide Set Includes All Five Colored Slides
  • 25.4 mm x 76.2 mm and 1.7 ± 0.2 mm Thick
  • Compatible with Our Sample Holders for 26 mm x 76 mm Slides Up to 1.7 mm Thick
  • Engraved Storage Box Included with FSK5 Slide Set and Packs of Five

Our Fluorescent Calibration Slides are designed to be used to spatially calibrate fluorescence imaging systems. Commonly used with confocal and two-photon imaging systems, these slides provide a uniform fluorescent field across their surface area and have fluorescence spectra similar to a variety of fluorophores. Each slide is 25.4 mm x 76.2 mm and has a thickness of 1.7 mm. A plastic storage box is included with the FSK5 Slide Set and the packs of five slides to keep them clean and dust-free. The box is engraved with the Thorlabs logo and item # on the top, as shown in the image to the right. For custom sizes up to 1' x 2', please contact Tech Support.

These slides are compatible with our sample holders that fit 26 mm x 76 mm slides up to 1.7 mm thick. Please note that our C4SH01 Four-Position Microscope Slide Holder is not compatible with these slides. 

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
FSK1 Support Documentation
FSK1NEW!Fluorescent Calibration Microscope Slide, Blue, 1.7 mm Thick
$5.00
Today
FSK1-P5 Support Documentation
FSK1-P5NEW!Fluorescent Calibration Microscope Slide, Blue, 1.7 mm Thick, 5 Pack
$18.00
Today
FSK2 Support Documentation
FSK2NEW!Fluorescent Calibration Microscope Slide, Green, 1.7 mm Thick
$5.00
Today
FSK2-P5 Support Documentation
FSK2-P5NEW!Fluorescent Calibration Microscope Slide, Green, 1.7 mm Thick, 5 Pack
$18.00
Today
FSK3 Support Documentation
FSK3NEW!Fluorescent Calibration Microscope Slide, Yellow, 1.7 mm Thick
$5.00
Today
FSK3-P5 Support Documentation
FSK3-P5NEW!Fluorescent Calibration Microscope Slide, Yellow, 1.7 mm Thick, 5 Pack
$18.00
Today
FSK4 Support Documentation
FSK4NEW!Fluorescent Calibration Microscope Slide, Orange, 1.7 mm Thick
$5.00
Today
FSK4-P5 Support Documentation
FSK4-P5NEW!Fluorescent Calibration Microscope Slide, Orange, 1.7 mm Thick, 5 Pack
$18.00
Today
FSK6 Support Documentation
FSK6NEW! Fluorescent Calibration Microscope Slide, Red, 1.7 mm Thick
$5.00
Today
FSK6-P5 Support Documentation
FSK6-P5NEW!Fluorescent Calibration Microscope Slide, Red, 1.7 mm Thick, 5 Pack
$18.00
Today
FSK5 Support Documentation
FSK5Fluorescent Calibration Microscope Slides, Set of 5 Colors
$18.54
Today

Fluorescent Alignment Disks


Click to Enlarge
The ADF5 Red Fluorescent Disk Mounted in an LMR1 Mount
  • Fluorescent Disks Available in Five Colors: Blue, Green, Yellow, Orange, and Red
    • Each Disk Sold Individually or in Packs of Five
  • Ø1" and 1.7 ± 0.2 mm Thick
  • Engraved with Target Guide Lines, and Ø3.4 mm and Ø9.4 mm Concentric Circles
  • Compatible with Our Ø1" Fixed Optic Mounts or Lens Tubes

Our Fluorescent Alignment Disks are designed to align fluorescence imaging systems. They have fluorescence spectra similar to various fluorophores, making them ideal for use with a variety of lasers used in fluorescence imaging. Each disk is 1" in diameter and has a thickness of 1.7 mm. A target with two concentric circles is engraved on every disk for easy alignment. For custom sizes up to 1' x 2', please contact Tech Support.

These disks can be mounted with our Ø1" fixed optic mounts or lens tubes, as shown in the image to the right. 

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
ADF1 Support Documentation
ADF1NEW!Fluorescent Alignment Disk, Blue
$8.00
Today
ADF1-P5 Support Documentation
ADF1-P5NEW!Fluorescent Alignment Disk, Blue, 5 Pack
$30.00
Today
ADF2 Support Documentation
ADF2NEW!Fluorescent Alignment Disk, Green
$8.00
Today
ADF2-P5 Support Documentation
ADF2-P5NEW!Fluorescent Alignment Disk, Green, 5 Pack
$30.00
Today
ADF3 Support Documentation
ADF3NEW!Fluorescent Alignment Disk, Yellow
$8.00
Today
ADF3-P5 Support Documentation
ADF3-P5NEW!Fluorescent Alignment Disk, Yellow, 5 Pack
$30.00
Today
ADF4 Support Documentation
ADF4NEW!Fluorescent Alignment Disk, Orange
$8.00
Today
ADF4-P5 Support Documentation
ADF4-P5NEW!Fluorescent Alignment Disk, Orange, 5 Pack
$30.00
Today
ADF5 Support Documentation
ADF5NEW!Fluorescent Alignment Disk, Red
$8.00
Today
ADF5-P5 Support Documentation
ADF5-P5NEW!Fluorescent Alignment Disk, Red, 5 Pack
$30.00
Today
Log In  |   My Account  |   Contact Us  |   Careers  |   Privacy Policy  |   Home  |   FAQ  |   Site Index
Regional Websites:East Coast US | West Coast US | Asia | China | Japan
Copyright 1999-2019 Thorlabs, Inc.
Sales: 1-973-300-3000
Technical Support: 1-973-300-3000


High Quality Thorlabs Logo 1000px:Save this Image