Customizable Optogenetics Kits

Complete Fiber Optics Kit for Optogenetics with Optional Component Substitution
470 nm LED Light Source
Ø1.25 mm or Ø2.5 mm Ferrule Cannulae with Ø200 μm or Ø400 μm Fiber Core
Standard and Rotary Joint Patch Cables

Kit with Rotary Joint Patch Cable, Ø200 µm Core Fiber, and Ø2.5 mm Ferrules

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Overview
Specs
User Guide
Fiber Cleaving
Feedback
OG Selection Guide

Kit #	Kit 1		Kit 2		Kit 3		Kit 4
Light Source	M470L5 Mounted 470 nm LED (Fiber-Coupled 590 nm Substitution Available)
LED Driver	LEDD1B
Fiber Core Size	Ø200 µm				Ø400 µm
Cannula Ferrule Size	Ø2.5 mm		Ø1.25 mm		Ø2.5 mm		Ø1.25 mm
Patch Cable	Standard	Rotary Joint	Standard	Rotary Joint	Standard	Rotary Joint	Standard	Rotary Joint
Cannula Output Power^a	2.6 mW	1.9 mW	4.5 mW	2.8 mW	17.9 mW	7.7 mW	18.8 mW	9.09 mW
Variation in Output Power^b	N/A	±7%	N/A	±7%	N/A	±7%	N/A	±7%

Approximate value, tested with the LED driven at maximum current (1 A).
Output power variation during rotation of the rotary joint.

Features

Complete Fiber Optics Setup Including LED, Patch Cable, Cannulae, and Accessories
Flexible Component Substitution
Stainless Steel Ferrules and Fiber Cores in Two Sizes:

Ø1.25 mm Ferrules (Ø200 or Ø400µm Core Fiber)
Ø2.5 mm Ferrules (Ø200 or Ø400 µm Core Fiber)

Standard Lightweight Patch Cable with Rotary Joint Patch Cable Option
Cannula Holders for Implantation with Stereotaxic Equipment
Fiber Cleaving and Cleaning Essentials
Cleaved Cannulae of Various Lengths with Uncleaved Cannulae Kit Option
Cannula Connectors
- 1 Quick-Release Interconnect
- 5 Mating Sleeves

Thorlabs' optogenetics equipment is available in complete, ready-to-use kits. Our optogenetics kits include a 470 nm Mounted LED with driver, lightweight patch cable, rotary joint patch cable, quick-release interconnect, five mating sleeves, and 20 stainless steel ferrule cannulae. Certain kits feature an additional 20 uncleaved cannula and fiber cleaving tools. Please see the Fiber Cleaving for an introduction to their use. Kits are available with Ø200 µm or Ø400 µm core fiber components with 0.39 NA. Ø200 µm core size cables are less invasive, making them ideal for smaller animals. Ø400 µm core size cables offer a stiffer, more robust solution for larger animals and higher power light sources. These kits also include accessories for cannula implantation, as well as fiber cleaning supplies to cover basic fiber care and usage.

Each component in these kits can be removed or substituted for items more applicable to the individual needs of the experiment. See below for the most relevant kit, and the OG Selection Guide for alternate cannula and patch cable options. Thorlabs also sells other fiber-coupled LEDs and fiber care supplies, including a fiber polishing and repair kit.

For more particular experimental needs, Thorlabs offers custom fiber components for optogenetics. For more information, contact Tech Support.

Optogenetics Product Family for In Vivo Applications

Thorlabs offers a wide variety of products designed to support in vivo optogenetics applications. Please visit the OG Selection Guide tab above to see a full listing of available products for different applications.

Thorlabs Optogenetics Equipment Selection Guide
Fiber Optic Cannulae	Rotary Joint Patch Cables	1x2 Rotary Joint Splitter	Standard Patch Cables	Interconnect and Mating Sleeves	Multimode Fiber Couplers	LED Light Sources	Laser Light Source	Customizable Kits

Optogenetics Specifications

Kit #	Kit 1		Kit 2		Kit 3		Kit 4
Light Source	M470L5 470 nm Mounted LED
LED Driver	LEDD1B
Fiber Type	FT200EMT				FT400EMT
Fiber Core Size	Ø200 µm				Ø400 µm
Fiber NA	0.39				0.39
Fiber Wavelength Range (Click for Plot)	400 - 2200 nm				400 - 2200 nm
Cannula Ferrule Size	Ø2.5 mm		Ø1.25 mm		Ø2.5 mm		Ø1.25 mm
Patch Cable	M77L01 (1 m Long)	RJPSF2 (3 m Long with Rotary Joint)	M89L01 (1 m Long)	RJPSL2 (3 m Long with Rotary Joint)	M79L01 (1 m Long)	RJPSF4 (3 m Long with Rotary Joint)	M98L01 (1 m Long)	RJPSL4 (3m Long with Rotary Joint)
Cannula Interconnects and Mating Sleeves	1 x ADAF2 Interconnect 1 x ADAF1-5 Mating Sleeves (5 Pack)		1 x ADAL3 Interconnect 1 x ADAL1-5 Mating Sleeves (5 Pack)		1 x ADAF2 Interconnect 1 x ADAF1-5 Mating Sleeves (5 Pack)		1 x ADAL3 Interconnect 1 x ADAL1-5 Mating Sleeves (5 Pack)
Cannulae	5 x CFM12L02 (2 mm) 5 x CFM12L05 (5 mm) 5 x CFM12L10 (10 mm) 5 x CFM12L20 (20 mm) 20 x CFM12U-20 (Uncleaved)		5 x CFML12L02 (2 mm) 5 x CFML12L05 (5 mm) 5 x CFML12L10 (10 mm) 5 x CFML12L20 (20 mm) 20 x CFML12U-20 (Uncleaved)		5 x CFM14L02 (2 mm) 5 x CFM14L05 (5 mm) 5 x CFM14L10 (10 mm) 5 x CFM14L20 (20 mm)		5 x CFML14L02 (2 mm) 5 x CFML14L05 (5 mm) 5 x CFML14L10 (10 mm) 5 x CFML14L20 (20 mm)
Approx. Cannula Output Power^a	2.6 mW	1.9 mW	4.5 mW	2.8 mW	17.9 mW	7.7 mW	18.8 mW	9.09 mW
Variation in Output Power^b	N/A	±7%	N/A	±7%	N/A	±7%	N/A	±7%

Tested with the LED driven at maximum current (1 A).
Output power variation during rotation of the rotary joint.

LED Specifications^a

Item #	M470L5
Center Wavelength	470 nm
Bandwidth (FWHM)	28 nm
Typical Output Spectrum (Click to Enlarge)
CW Drive Current (Max)	1 A
LED Forward Voltage	3.8 V
Typical Lifetime	>100,000 Hours

Please refer to the M470L5 web presentation or spec sheet for complete specifications.

Fiber Specifications

Fiber Type	FT200EMT Multimode	FT400EMT Multimode
Core Diameter	Ø200 ± 5 µm	Ø400 ± 8 µm
Wavelength	400-2200 nm
NA	0.39 ± 0.02
Core / Cladding	Pure Silica / TECS Hard Cladding
Cladding Diameter	225 ± 5 μm	425 ± 10 μm
Coating Diameter	500 ± 30 μm	730 ± 30 μm
Max Core Offset	5 µm	7 µm
Bend Radius (Short Term / Long Term)	9 mm / 18 mm	20 mm / 40 mm

LED Driver Specifications

Item #	LEDD1B^a
Common Data
Output Current Range	0 - 1200 mA
LED Current Limit Set Point Range	200 - 1200 mA
LED Forward Voltage	11 V (Min) 12 V (Typical)
Current Ripple	8 mA
Current Ripple Frequency	570 kHz
Modulation Mode^b
Frequency Range	0 - 5 kHz, Sine Wave
Modulation Form	Arbitrary
Input Voltage Rate	0 - 5 V
Slew Rate	13.6 mA/µs
Decay Rate	13.1 mA/µs
Trigger Mode^b
Frequency Range	0 - 1 kHz
Duty Cycle Range	20 - 80% @ 1 kHz 2 - 98% @ 100 Hz 0.2 - 99.8% @ 10 Hz
Modulation Form	Square Wave / PWM
Logic Input Levels	TTL Min H-Level: 2 V Max L-Level: 0.55 V
Slew Rate	18 mA/µs
Rise Time	51 µs
Decay Rate	12 mA/µs
Fall Time	79 µs
General Data
Power Supply	15 VDC
Maximum Power Consumption	15 VA
Operating Temperature	0 - 40 °C
Storage Temperature	-40 to 70 °C
Weight	240 g
Dimensions (W x H x D)	60 mm x 73 mm x 104 mm

All the technical data is valid at 23 ± 5 °C and 45 ± 15% relative humidity.
Specifications for the modulation and trigger modes depend on the forward voltage and capacitance of the connected LED.

Figure 1. Adjust the LEDD1B current limit adjuster arrow with a flathead screwdriver to set the maximum current (1.0 A or less).

Setup

LED and Driver
All Optogenetics Starter Kits include a Mounted or Fiber-Coupled LED and an LED Driver (Item # LEDD1B). For the mounted LED M470L5, the included SM1L05 lens tube should be threaded on to the internal threads of the LED. The SM1SMA fiber adapter should then be threaded into the lens tube until it contacts the end of the LED. To avoid damaging the LED, do not force the SM1SMA farther into the lens tube after it makes contact with the LED. In order to prevent the adapter from being driven into the LED during attachment of patch cables, the retaining ring included with the lens tube can be threaded in to the appropriate location before adding the adapter.

The LED can be operated by connecting it to the “LED” jack in the back of the LED driver. Plug the power supply (Item # KPS201) into the LED driver, and then the power supply to the main line voltage. The LED can only be driven up to a maximum current of 1.0 A without permanent damage; use a flathead screwdriver to turn the directional arrow recessed in the current limit adjuster on the front of the driver to set the maximum driving current (see image to the right). The LED driver can then be powered on by turning the knob on top of the driver clockwise.

Patch Cable and Cannula
Insert the patch cable’s SMA connector into the LED unit by threading the connector’s rotating barrel onto the LED unit’s housing. Then, depending upon your choice of interconnect or mating sleeve, the connection methodology varies.

If you are using an ADAL1 (Ø1.25 mm ferrules) or ADAF1 (Ø2.5 mm ferrules) mating sleeve, place the mating sleeve onto the ferrule end of the patch cable. Leave approximately one-third of the mating sleeve length exposed for the cannula connection (Figure 2). Then, connect the mating sleeve to the cannula. To disconnect the cannula, grip the patch cable by the ferrule and mating sleeve and use a twisting motion. It is very important that the ends of the patch cable’s ferrule and the cannula’s ferrule are in physical contact (Figure 3). If they are not, the output power at the cannula tip will decrease significantly (Figure 4).

ADAF1 Mating Sleeve and Ferrule Patch Cable

Click to Enlarge
Figure 2. The ADAF1 Mating Sleeve should be inserted 1/2 to 2/3 of the way onto the patch cable before cannula connection.

Click to Enlarge
Figure 3. Properly connected cannula and patch cable, with the cannula and patch cable in physical contact.

Fiber Optic Connector Improperly Connected

Click to Enlarge
Figure 4. Improperly connected cannula and patch cable, showing an air gap and light leakage.

Quick-Release Interconnect Usage

The ADAF2 (Ø2.5 mm ferrules) quick-release interconnect provides a more reliable mating method for cannulae and patch cables. First, insert a patch cable ferrule into the interconnect and secure by tightening the setscrew using the included hex key [0.05" (1.3 mm)]. Then, squeeze the quick-release lever and insert the cannula until it makes physical contact with the ferrule. Finally, release the lever to lock the cannula into place. The interconnect is engraved to indicate where squeeze pressure should be applied. To release the cannula (or disconnect from an implanted cannula), squeeze the quick-release lever and pull the interconnect away from the cannula; only light force is needed to disconnect the interconnect. See the animation on the right for a visual depiction of this process.

Rotary Joint Patch Cables
These cables operate exactly the same way as standard patch cables, but they have an articulated joint that prevents tangling from specimen movement. SM05 (0.535"-40) mounting threads are present on the light source side of the joint. The joint can be mounted using Thorlabs’ extensive line of SM05-compatible optomechanics hardware, such as the LMR05 lens mount. Alternatively, the rotary joint can be mounted directly to the wall or ceiling of a specimen enclosure. An SM05-tapped hole can be created using Thorlabs’ 83373 SM05 tap, or the joint can be glued into a ~Ø1/2" hole. Click here for more information about mounting the rotary joint.

Operation

Make sure the current limit on the LED driver is set to 1.0 A or below before operating the LED to avoid permanent damage.

The LED can be operated using the LED driver only, or with the LED driver and an external signal. It can be operated in one of the following modes: Continuous Wave (CW), Tigger (TRIG), or Modulation (MOD). For more information on the LEDD1B driver's operation, see the full web presentation and manual.

Continuous Wave (CW) Mode
While operating in continuous wave mode, the driver provides a constant non-modulated LED current. The user controls the LED’s power by adjusting the control knob.

Trigger (TRIG) Mode
This mode operates using an externally applied TTL signal to pulse the LED output. The width of the TTL signal can be used to define the LED pulse width as well. The brightness of the LED is adjusted using the control knob.

Modulation (MOD) Mode
This mode allows the LED driver to be controlled completely by an external voltage. LED intensity varies with the input voltage, from off (0V) to maximum brightness (5V).

Fiber Care

Patch cable connectors should be cleaned every time the cable is mated, tested, or reconfigured. Clean the fiber endface using a lint-free wipe (Item # LFW90 in the CKF fiber cleaning kit) that has been wetted with connector cleaner (Item # FCS3 in the CKF fiber cleaning kit), then inspect the fiber with the inspection scope (Item # FS201). Bare fiber should be cleaned by wrapping the damp wipe around the fiber, squeezing gently, and sliding the wipe toward the end of the fiber until it squeaks. Inspect the fiber end with either the inspection scope (Item # FS201) or eye loupe (Item # JEL10). See the fiber cleaning kit spec sheet for details.

Fiber Cleaving

Uncleaved cannula must be cleaved, or precision cut, before use. A high-quality fiber cleave will preserve the fidelity of the optical signal transmitted through the tip of the fiber. We recommend the following procedure for cleaving the fiber end.

Required Materials

Uncleaved Fiber Optic Cannula*
Marker
S90R Fiber Scribe*
BFG1 Bare Fiber Gripper*
KW32 Kimwipes^®† Lint-Free Wipes
Isopropyl Alcohol or FCS3 Fiber Cleaning Fluid*
JEL10 Eye Loupe* or Microscope
FTDU Fiber Optic Disposal Unit*
Safety Goggles/Glasses

Starred (*) items are provided in kits with uncleaved cannulae.

Cleaving Procedure

Clean the fiber end of the cannula using a lint-free wipe and isopropyl alcohol or FCS3 fiber cleaning fluid. Do not use acetone, as this will damage the TECS cladding on the exposed fiber.
Measure the desired length of fiber from the end of the ferrule. Mark this length on the fiber with a marker.
Place the cannula on a hard surface and secure the fiber end with a finger or tape, as shown in Figure 1.
Hold the cleaving tool perpendicular to the fiber and gently score the fiber as shown in Figure 1 to the right. Do not apply excessive pressure. The fiber should not break off at this point. This step is critical in obtaining a good cleave. If the scribe is made too hard, the fiber will break instead of cleaving. If the scribe is too light, the fiber will not cleave.
Hold the cannula in one hand, and grip the fiber end with a bare fiber gripper in the other end as shown in Figure 2. Pull the fiber straight back until the fiber cleaves as shown in Figure 3. Dispose of the scrap fiber end in the FTDU or any medical or fiber sharps container.
If the fiber does not break with a moderate amount of tension, repeat steps 4-5 above applying slightly more pressure when scoring. Inspect the cleave using an eye loupe or microscope.

A good cleave will be flat across the fiber and perpendicular to the optic axis. There should be no ‘tag’ (i.e., protrusion) from the edge of the fiber. The region where the initial scribe was made may be visible. It should be less than 5% of the core diameter. Be patient as this process takes a little practice. Please be aware that it will be more difficult to achieve a high-quality cleave in large-core-diameter fibers compared to thinner fibers. A view of a properly cleaved fiber end as seen through a Thorlabs' JEL10 eye loupe is shown in Figure 4.

Click Here for a Print Friendly Version of this Procedure

Thorlabs also offers a full fiber termination manual, which covers fiber termination and bare fiber cleaving. It can be downloaded for free here, or a paper copy can be purchased here.

^†Kimwipes^® is a registered trademark of the Kimberly-Clark Corporation.

Posted Comments:

user (posted 2013-04-08 19:24:12.54)

What ever happened to your rotary joint. I think there used to be a page or at least an image of a finished unit. I really need one for my research

tcohen (posted 2013-04-09 11:04:00.0)

Response from Tim at Thorlabs: Thank you for your interest. The rotary joint went through a redesign so that we could provide the best quality before initial release. We have plans to release four different units within the next couple of weeks and we will be adding supporting documentation to the website upon release. If you would like to discuss the specs, timeline or fit within your application, please contact us at techsupport@thorlabs.com.

user (posted 2013-04-05 21:45:22.623)

when will a kit with rotary joint be available?

Quick Links
Single-Site Stimulation
One Light Source to One Cannula Implant
Bilateral Stimulation
One Light Source to Two Cannula Implants Using Rotary Joint Splitter
One or Two Light Sources to Two Cannula Implants
Two Light Sources into One Dual-Core Cannula Implant
Illumination
Fiber-Coupled LEDs and Drivers

Optogenetics Selection Guide

Thorlabs offers a wide range of optogenetics components; the compatibility of these products in select standard configurations is discussed in detail here. Please contact Technical Support for assistance with items outside the scope of this guide, including custom fiber components for optogenetics.

Single-Site Stimulation

One Light Source to One Cannula Implant

The most straightforward method for in vivo light stimulation of a specimen is to use a single fiber optic with a single LED light source. The single wavelength LED is powered by an LED driver, and then the illumination output is fiber-coupled into a patch cable, which connects to the implanted cannula. See the graphics and expandable compatibility tables below for the necessary patch cables and cannulae to create this setup. To choose the appropriate LED and driver, see below or the full web presentation.

Click on Each Component for More Information

Click for Details

Click to See Ø1.25 mm (LC) Ferrule Compatible Patch Cables, Cannulae, and Interconnects

Component Selection Table (Click Links for Item Description Popup)
Fiber Type		FG105LCA	FG200UCC	FT200EMT	FP200URT	FT400EMT	FP400URT
Fiber Type Specs	Core Diameter	105 µm	200 µm			400 µm
	Wavelength Range	400 - 2400 nm	250 - 1200 nm	400 - 2200 nm	300 - 1200 nm	400 - 2200 nm	300 - 1200 nm
	NA	0.22	0.22	0.39	0.50	0.39	0.50
Patch Cables^b	Standard FC/PC Input	M61L01	M86L005 M86L01	M83L01 MR83L01	M73L01	M99L01	M127L01
	Standard SMA905 Input	M63L01	M87L005 M87L01	M89L01 MR89L01	M95L01	M98L01	M125L01
	Rotary Joint FC/PC Input	-	-	RJPFL2	-	RJPFL4	-
	Rotary Joint SMA905 Input	-	-	RJPSL2	-	RJPSL4	-
Compatible Mating Sleeve/Interconnect		ADAL1, ADAL3, or ADAL4-5
Fiber Optic Cannulae^c	Ceramic 6.4 mm Long Ferrule	CFMLC21L02 CFMLC21L05 CFMLC21L10 CFMLC21L20 CFMLC21U-20	CFMLC22L02 CFMLC22L05 CFMLC22L10 CFMLC22L20 CFMLC22U-20	CFMLC12L02 CFMLC12L05 CFMLC12L10 CFMLC12L20 CFMLC12U-20	CFMLC52L02 CFMLC52L05 CFMLC52L10 CFMLC52L20 CFMLC52U-20	CFMLC14L02 CFMLC14L05 CFMLC14L10 CFMLC14L20 CFMLC14U-20	CFMLC15L02 CFMLC15L05 CFMLC15L10 CFMLC15L20
	Diffuser Tip (Ceramic 6.4 mm Long Ferrule)	-	-	CFDSB02 CFDSB05 CFDSB10 CFDSB20	-	-	-
	Ceramic 10.5 mm Long Ferrule	-	CFMXA02 CFMXA05 CFMXA10 CFMXA20	CFMXB02 CFMXB05 CFMXB10 CFMXB20	CFMXC02 CFMXC05 CFMXC10 CFMXC20	-	CFMXD02 CFMXD05 CFMXD10 CFMXD20
	Stainless Steel 6.4 mm Long Ferrule	CFML21L02 CFML21L05 CFML21L10 CFML21L20 CFML21U-20	CFML22L02 CFML22L05 CFML22L10 CFML22L20 CFML22U-20	CFML12L02 CFML12L05 CFML12L10 CFML12L20 CFML12U-20	CFML52L02 CFML52L05 CFML52L10 CFML52L20 CFML52U-20	CFML14L02 CFML14L05 CFML14L10 CFML14L20 CFML14U-20	CFML15L02 CFML15L05 CFML15L10 CFML15L20
Items are compatible with each other when they are comprised of the same fiber type and listed in the same column. Patch cables for single source to single implant applications are highlighted in green above. Choose a patch cable with an input that matches your light source. Available cannulae are highlighted in orange of the table above. Cannulae within the same column are interchangeable.

Click for Detials

Click to See Ø2.5 mm (FC) Ferrule Compatible Patch Cables, Cannulae, and Interconnects

Component Selection Table (Click Links for Item Description Popup)
Fiber Type		FG200UCC	FT200EMT	FP200URT	FT300EMT	FT400EMT	FP400URT
Fiber Type Specs	Core Diameter	200 µm			300 µm	400 µm
	Wavelength Range	250 - 1200 nm	400 - 2200 nm	300 - 1200 nm	400 - 2200 nm	400 - 2200 nm	300 - 1200 nm
	NA	0.22	0.39	0.50	0.39	0.39	0.50
Patch Cables^b	Standard FC/PC Input	M80L005 M80L01	M81L005 M81L01 MR81L01	M104L01	M56L005 M56L01	M82L005 M82L01	M128L01
	Standard SMA905 Input	M84L005 M84L01	M77L005 M77L01 MR77L01	M106L01	M58L005 M58L01	M79L005 M79L01	M126L01
	Rotary Joint FC/PC Input	-	RJAFF2 RJPFF2	-	-	RJPFF4	-
	Rotary Joint SMA905 Input	-	RJASF2 RJPSF2	-	-	RJPSF4	-
Compatible Mating Sleeve/Interconnect		ADAF1, ADAF2, or ADAF4-5
Fiber Optic Cannulae^c	Ceramic 10.5 mm Long Ferrule	CFMC22L02 CFMC22L05 CFMC22L10 CFMC22L20 CFMC22U-20	CFMC12L02 CFMC12L05 CFMC12L10 CFMC12L20 CFMC12U-20	CFMC52L02 CFMC52L05 CFMC52L10 CFMC52L20 CFMC52U-20	CFMC13L02 CFMC13L05 CFMC13L10 CFMC13L20	CFMC14L02 CFMC14L05 CFMC14L10 CFMC14L20 CFMC14U-20	CFMC54L02 CFMC54L05 CFMC54L10 CFMC54L20
Fiber Optic Cannulae^c	Stainless Steel 12.7 mm Long Ferrule	CFM22L02 CFM22L05 CFM22L10 CFM22L20 CFM22U-20	CFM12L02 CFM12L05 CFM12L10 CFM12L20 CFM12U-20	CFM52L02 CFM52L05 CFM52L10 CFM52L20 CFM52U-20	CFM13L02 CFM13L05 CFM13L10 CFM13L20	CFM14L02 CFM14L05 CFM14L10 CFM14L20 CFM14U-20	CFM15L02 CFM15L05 CFM15L10 CFM15L20
Items are compatible with each other when they are comprised of the same fiber type and listed in the same column. Patch cables for single source to single implant applications are highlighted in green above. Choose a patch cable with an input that matches your light source. Available cannulae are highlighted in orange of the table above. Cannulae within the same column are interchangeable.

Bilateral Stimulation

The ability to accurately and simultaneously direct light to multiple locations within a specimen is desired for many types of optogenetics experiments. For example, bilateral stimulation techniques typically target neurons in two spatially separated regions in order to induce a desired behavior. In more complex experiments involving the simultaneous inhibition and stimulation of neurons, delivering light of two different monochromatic wavelengths within close proximity enables the user to perform these experiments without implanting multiple cannulae, which can increase stress on the specimen.

Bilateral stimulation can be achieved with several different configurations depending on the application requirements. The sections below illustrate examples of different configurations using Thorlabs' optogenetics products.

Option 1: One Light Source to Two Cannula Implants Using Rotary Joint Splitter

Thorlabs' RJ2 1x2 Rotary Joint Splitter is designed for optogenetics applications and is used to split light from a single input evenly between two outputs. The rotary joint interface allows connected patch cables to freely rotate, reducing the risk of fiber damage caused by a moving specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.

Click for Details

Click to See Ø1.25 mm (LC) Ferrule Components Recommended for Use with RJ2 Rotary Joint Splitter

Component Selection Table (Click Links for Item Description Popup)
Common Fiber Properties
Fiber Properties	Core Diameter	200 µm			400 µm
	Wavelength Range^a	400 - 700 nm	400 - 700 nm	400 - 700 nm	400 - 700 nm	400 - 700 nm
	NA	0.22	0.39	0.50	0.39	0.50
Input Patch Cables^b	Standard FC/PC Connectors	M122L01 M122L02 M122L05	M72L01 M72L02 M72L05	M123L01 M123L02	M74L01 M74L02 M74L05	M124L01 M124L02
Input Patch Cables^b	Hybrid SMA905 to FC/PC	M36L01	M75L01 M75L02	M129L01 M129L02	M76L01 M76L02	M131L01 M131L02
Rotary Joint		RJ2
Output Patch Cables	FC/PC to Ø1.25 mm (LC) Ferrule	M86L005 M86L01	M83L01 MR83L01	M73L01	M99L01	M127L01
Compatible Mating Sleeve/Interconnect		ADAL1, ADAL3, or ADAL4-5
Fiber Optic Cannulae^c	Ceramic 6.4 mm Long Ferrule	CFMLC22L02 CFMLC22L05 CFMLC22L10 CFMLC22L20 CFMLC22U-20	CFMLC12L02 CFMLC12L05 CFMLC12L10 CFMLC12L20 CFMLC12U-20	CFMLC52L02 CFMLC52L05 CFMLC52L10 CFMLC52L20 CFMLC52U-20	CFMLC14L02 CFMLC14L05 CFMLC14L10 CFMLC14L20 CFMLC14U-20	CFMLC15L02 CFMLC15L05 CFMLC15L10 CFMLC15L20
	Diffuser Tip (Ceramic 6.4 mm Long Ferrule)	-	CFDSB02 CFDSB05 CFDSB10 CFDSB20	-	-	-
	Ceramic 10.5 mm Long Ferrule	CFMXA02 CFMXA05 CFMXA10 CFMXA20	CFMXB02 CFMXB05 CFMXB10 CFMXB20	CFMXC02 CFMXC05 CFMXC10 CFMXC20	-	CFMXD02 CFMXD05 CFMXD10 CFMXD20
	Stainless Steel 6.4 mm Long Ferrule	CFML22L02 CFML22L05 CFML22L10 CFML22L20 CFML22U-20	CFML12L02 CFML12L05 CFML12L10 CFML12L20 CFML12U-20	CFML52L02 CFML52L05 CFML52L10 CFML52L20 CFML52U-20	CFML14L02 CFML14L05 CFML14L10 CFML14L20 CFML14U-20	CFML15L02 CFML15L05 CFML15L10 CFML15L20
Products in each column can be used with each other over the indicated wavelength range and is limited by the operating range of the RJ2 Rotary Joint Splitter. Individual products may be used outside this range; see product information for detailed specifications. Use a standard FC/PC patch cable to connect to an FC/PC-terminated light source. SMA905 to FC/PC hybrid patch cables should be used to connect to an SMA905-terminated light source. Available cannulae are highlighted in orange of the table above. Cannulae within the same column are interchangeable.

Click for Detials

Click to See Ø2.5 mm (FC) Ferrule Components Recommended for Use with RJ2 Rotary Joint Splitter

Component Selection Table (Click Links for Item Description Popup)
Common Fiber Properties
Fiber Properties	Core Diameter	200 µm			300 µm	400 µm
	Wavelength Range^a	400 - 700 nm	400 - 700 nm	400 - 700 nm	400 - 700 nm	400 - 700 nm	400 - 700 nm
	NA	0.22	0.39	0.50	0.39	0.39	0.50
Input Patch Cables	Standard FC/PC Connectors	M122L01 M122L02 M122L05	M72L01 M72L02 M72L05	M123L01 M123L02	M56L005 M56L01	M74L01 M74L02 M74L05	M124L01 M124L02
Input Patch Cables	Hybrid SMA905 to FC/PC	M36L01	M75L01 M75L02	M129L01 M129L02	M58L005 M58L01	M76L01 M76L02	M131L01 M131L02
Rotary Joint		RJ2
Output Patch Cables	FC/PC to Ø2.5 mm (FC) Ferrule	M80L005 M80L01	M81L005 M81L01 MR81L01	M104L01	M56L005 M56L01	M82L005 M82L01	M128L01
Compatible Mating Sleeve/Interconnect		ADAF1, ADAF2, or ADAF4-5
Fiber Optic Cannulae^c	Ceramic 10.5 mm Long Ferrule	CFMC22L02 CFMC22L05 CFMC22L10 CFMC22L20 CFMC22U-20	CFMC12L02 CFMC12L05 CFMC12L10 CFMC12L20 CFMC12U-20	CFMC52L02 CFMC52L05 CFMC52L10 CFMC52L20 CFMC52U-20	CFMC13L02 CFMC13L05 CFMC13L10 CFMC13L20	CFMC14L02 CFMC14L05 CFMC14L10 CFMC14L20 CFMC14U-20	CFMC54L02 CFMC54L05 CFMC54L10 CFMC54L20
Fiber Optic Cannulae^c	Stainless Steel 12.7 mm Long Ferrule	CFM22L02 CFM22L05 CFM22L10 CFM22L20 CFM22U-20	CFM12L02 CFM12L05 CFM12L10 CFM12L20 CFM12U-20	CFM52L02 CFM52L05 CFM52L10 CFM52L20 CFM52U-20	CFM13L02 CFM13L05 CFM13L10 CFM13L20	CFM14L02 CFM14L05 CFM14L10 CFM14L20 CFM14U-20	CFM15L02 CFM15L05 CFM15L10 CFM15L20
Products in each column can be used with each other over the indicated wavelength range and is limited by the operating range of the RJ2 Rotary Joint Splitter. Individual products may be used outside this range; see product information for detailed specifications. Use a standard FC/PC patch cable to connect to an FC/PC-terminated light source. SMA905 to FC/PC hybrid patch cables should be used to connect to an SMA905-terminated light source. Available cannulae are highlighted in orange of the table above. Cannulae within the same column are interchangeable.

Option 2: One or Two Light Sources to Two Cannula Implants

If the intent is for one LED source to connect to two cannulae for simultaneous light modulation, then a bifurcated fiber bundle can be used to split the light from the LED into each respective cannula. For dual wavelength stimulation (mixing two wavelengths in a single cannula) or a more controlled split ratio between cannula, one can use a multimode coupler to connect one or two LEDs to the cannulae. If one cable end is left unused, the spare coupler cable end may be terminated by a light trap. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.

Click on Each Component Below for More Information

Click to see Compatible Fiber Bundles, Coupler/Splitters, Cannulae, and Interconnects

Part Selection Table (Click Links for Item Description Popup)
Common Fiber Properties
Core Diameter		105 µm	200 µm						400 µm
Wavelength Range		400 - 2200 nm^a	400 - 1200 nm^a		400 - 900 nm^a				400 - 2200 nm
NA		0.22	0.22		0.39		0.50		0.39
Ferrule Style^b		LC (Ø1.25 mm)	LC (Ø1.25 mm)	FC (Ø2.5 mm)	LC (Ø1.25 mm)	FC (Ø2.5 mm)	LC (Ø1.25 mm)	FC (Ø2.5 mm)	LC (Ø1.25 mm)	FC (Ø2.5 mm)
Bifurcated Y-Cable^c	FC/PC Input	-	BFYL1LF01	BFYF1LF01	BFYL2LF01	BFYF2LF01	-	-	BFYL4LF01	BFYF4LF01
Bifurcated Y-Cable^c	SMA905 Input	-	BFYL1LS01	BFYF1LS01	BFYL2LS01	BFYF2LS01	-	-	BFYL4LS01	BFYF4LS01
1x2 Multimode Coupler^c	FC/PC Input	TM105FL1B	TM200FL1B	TM200FS1B	TT200FL1A	TT200FS1A	TH200FL1A	TH200FS1A	-	-
1x2 Multimode Coupler^c	SMA905 Input	TM105SL1B	TM200SL1B	TM200SS1B	TT200SL1A	TT200SS1A	TH200SL1A	TH200SS1A	-	-
2x2 Multimode Coupler^c	FC/PC Input	-	-	-	TT200FL2A	TT200FS2A	-	-	-	-
2x2 Multimode Coupler^c	SMA905 Input	-	-	-	TT200SL2A	TT200SS2A	-	-	-	-
Compatible Mating Sleeve/Interconnect		ADAL1 ADAL3 ADAL34-5	ADAL1 ADAL3 ADAL4-5	ADAF1 ADAF2 ADAF4-5	ADAL1 ADAL3 ADAL4-5	ADAF1 ADAF2 ADAF4-5	ADAL1 ADAL3 ADAL4-5	ADAF1 ADAF2 ADAF4-5	ADAL1 ADAL3 ADAL4-5	ADAF1 ADAF2 ADAF4-5
Fiber Optic Cannulae^d	Ceramic 6.4 mm Long Ferrule	CFMLC21L02 CFMLC21L05 CFMLC21L10 CFMLC21L20 CFMLC21U-20	CFMLC22L02 CFMLC22L05 CFMLC22L10 CFMLC22L20 CFMLC22U-20	-	CFMLC12L02 CFMLC12L05 CFMLC12L10 CFMLC12L20 CFMLC12U-20	-	CFMLC52L02 CFMLC52L05 CFMLC52L10 CFMLC52L20 CFMLC52U-20	-	CFMLC14L02 CFMLC14L05 CFMLC14L10 CFMLC14L20 CFMLC14U-20	-
	Diffuser Tip (Ceramic 6.4 mm Long Ferrule)	-	-	-	CFDSB02 CFDSB05 CFDSB10 CFDSB20	-	-	-	-	-
	Ceramic 10.5 mm Long Ferrule	-	CFMXA02 CFMXA05 CFMXA10 CFMXA20	CFMC22L02 CFMC22L05 CFMC22L10 CFMC22L20 CFMC22U-20	CFMXB02 CFMXB05 CFMXB10 CFMXB20	CFMC12L02 CFMC12L05 CFMC12L10 CFMC12L20 CFMC12U-20	CFMXC02 CFMXC05 CFMXC10 CFMXC20	CFMC52L02 CFMC52L05 CFMC52L10 CFMC52L20 CFMC52U-20	-	CFMC14L02 CFMC14L05 CFMC14L10 CFMC14L20 CFMC14U-20
	Stainless Steel	CFML21L02 CFML21L05 CFML21L10 CFML21L20 CFML21U-20	CFML22L02 CFML22L05 CFML22L10 CFML22L20 CFML22U-20	CFM22L02 CFM22L05 CFM22L10 CFM22L20 CFM22U-20	CFML12L02 CFML12L05 CFML12L10 CFML12L20 CFML12U-20	CFM12L02 CFM12L05 CFM12L10 CFM12L20 CFM12U-20	CFML52L02 CFML52L05 CFML52L10 CFML52L20 CFML52U-20	CFM52L02 CFM52L05 CFM52L10 CFM52L20 CFM52U-20	CFML14L02 CFML14L05 CFML14L10 CFML14L20 CFML14U-20	CFM14L02 CFM14L05 CFM14L10 CFM14L20 CFM14U-20

Products in this column can be used with each other over the indicated wavelength range. Individual products may be used outside this range; see product information for detailed specifications.
LC components have a Ø1.25 mm ferrule end while FC components have a Ø2.5 mm ferrule end. Items listed in the same "Ferrule Style" column are compatible with each other.
Optogenetics products for one or two light sources to two cannula implant applications are highlighted in green above. Choose a component with an input that matches your light source.
Available cannulae are highlighted in orange of the table above. Cannule within the same column are interchangeable.

Two Light Sources into One Dual-Core Cannula Implant

For bilateral stimulation applications where the two cannulas need to be placed in close proximity (within ~1 mm), Thorlabs offers dual-core patch cables and cannulae that are designed for this specific application. Each core is driven by a separate light source, enabling users to stimulate and/or supress nerve cells in the same region of the specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation.

Click on Each Component for More Information

Part Selection Table (Click Links for Item Description Popup)
Common Fiber Properties
Core Diameter		200 µm
Wavelength Range		400 - 2200 nm
NA		0.39
Fiber Type		FT200EMT
Ferrule Style^a		FC (Ø2.5 mm)
Dual-Core Patch Cable	FC/PC Input	BFY32FL1
Dual-Core Patch Cable	SMA905 Input	BFY32SL1
Compatible Mating Sleeve/Interconnect		ADAF1 ADAF2 ADAF4-5
Dual-Core Fiber Optic Cannulae^c	Stainless Steel	CFM32L10 CFM32L20

FC components have a Ø2.5 mm ferrule end.
Patch cables for dual light source to single implant applications are highlighted in green above. Choose a patch cable with an input that matches your light source.
Available cannulae are highlighted in orange of the table above. Cannule within the same column are interchangeable.

LED Item #	Wavelength^a	Typical Opsin	Output Power^b	Color
M385F1^c	385 nm	EBFP, moxBFP	10.7 mW	UV
M405F1^c	405 nm	mmilCFP, hcriGFP	3.7 mW	UV
M430F1	430 nm	ChR2	7.5 mW	Violet
M455F3	455 nm	ChIEF, bPAC	24.5 mW	Royal Blue
M505F3	505 nm	ChRGR, Opto-α1AR, Opto-β2AR	11.7 mW	Cyan
M530F2	530 nm	C1V1, VChR1	9.6 mW	Green
M565F3	565 nm	Arch, VChR1-SFO	13.5 mW	Lime
M595F2	595 nm	ChR2-SFO, eNpHR3.0	11.5 mW	Amber
M625F2	625 nm	ReChR	17.5 mW	Red

Click the link for a spectrum and raw data.
Typical output power measured with a Ø400 µm Core, 0.39 NA multimode fiber.
Precautions must be taken to prevent looking directly at the UV light and UV light protective glasses must be worn to avoid eye damage. Exposure of the skin and other body parts to UV light should be avoided.

Illumination

Click to Enlarge
M405F1

Fiber-Coupled LEDs and Drivers

Our fiber-coupled LEDs are ideal light sources for optogenetics applications. They feature a variety of wavelength choices and a convenient interconnection to optogenetics patch cables. Thorlabs offers fiber-coupled LEDs with nominal wavelengths ranging from 280 nm to 1050 nm. See the table to the right for the LEDs with the most popular wavelengths for optogenetics. A table of compatible LED drivers can be viewed by clicking below.

Click to See Compatible LED Drivers

Compatible Drivers^a	UPLED^b	LEDD1B^c	DC2200^b	DC4100^b,d,e	DC4104^b,d,e
Click Photos to Enlarge
Main Driver Features	USB-Controlled	Very Compact Footprint 60 mm x 73 mm x 104 mm (W x H x D)	Touchscreen Interface with Internal and External Options for Pulsed and Modulated LED Operation	4 Channels^d	4 Channels^d
LED Driver Current Output (Max)	1.2 A	1.2 A	LED1 Terminal: 10.0 A LED2 Terminal: 2.0 A^f	1.0 A per Channel	1.0 A per Channel
LED Driver Forward Voltage (Max)	8 V	12 V	50 V	5 V	5 V
Modulation Frequency	-	0 to 5 kHz (External)	20 to 100 kHz (Internal)^h DC to 250 kHz (External)^g,h	0 to 100 kHz (External)^h Simultaneous Across all Channels	0 to 100 kHz (External)^h Independently Controlled Channels
External Control Interface(s)	USB 2.0	Analog (BNC)	USB 2.0 and Analog (BNC)	USB 2.0 and Analog (BNC)	USB 2.0 and Analog (8-Pin)
EEPROM Compatible: Reads Out LED Data for LED Settings		-
LCD Display	-	-

Click on the item number link to view the complete presentation for each controller.
Automatically limits to LED's max current via EEPROM readout.
The preferred power supply (single channel or hub-based) depends on the end user's application and whether you already own compatible power supplies. To that end and in keeping with Thorlabs' green initiative, we do not ship the LEDD1B bundled with a power supply. This avoids the cost and inconvenience of receiving an unwanted single-channel supply if a hub-based system (Item # KCH301 or KCH601) would be more appropriate.
The DC4100 and DC4104 can power and control up to four LEDs simultaneously when used with the DC4100-HUB. The fiber-coupled LEDs on this page all require the DC4100-HUB when used with the DC4100 or DC4104.
These LED drivers have a maximum forward voltage rating of 5 V and can provide a maximum current of 1000 mA. As a result, they cannot be used to drive LEDs which have forward voltage ratings greater than 5 V. LEDs with maximum current ratings higher than 1.0 A can be driven using this driver, but will not reach full power.
The fiber-coupled LEDs sold above are compatible with the LED2 Terminal.
Small Signal Bandwidth: Modulation not exceeding 20% of full scale current. The driver accepts other waveforms, but the maximum frequency will be reduced.
The M565F3 and M595F2 LEDs produce light by stimulating emission from phosphor, which limits their modulation frequency range. These LEDs may not turn off completely when modulated above 10 kHz at duty cycles below 50%.

Kit 1: Ø200 µm Core Fiber, Ø2.5 mm Ferrules, and Rotary Joint Patch Cable

Item #	Product Description	Quantity	Alternate Items
Light Source
M470L5	470 nm Mounted LED	1	Alternate Fiber-Coupled LEDs^a
SM1L05	SM1-Threaded Lens Tube	1
SM1SMA	SMA Fiber Adapter	1
LEDD1B	LED Driver	1	Alternate LED Drivers
KPS201	LED Driver Power Supply	1	Alternate LED Drivers
Cannulae
CFM12L02	2 mm Length Cannula	5	Alternate Cannulae
CFM12L05	5 mm Length Cannula	5
CFM12L10	10 mm Length Cannula	5
CFM12L20	20 mm Length Cannula	5
CFM12U-20	Uncleaved Cannula, 20 Pack	1
Patch Cables and Cannula Connectors
M77L01	Standard Patch Cable	1	Alternate Patch Cables
RJPSF2	Rotary Joint Patch Cable	1	Alternate Patch Cables
ADAF2	Interconnect	1	Cannula Connectors
ADAF1-5	Mating Sleeves (Pack of Five)^b	1	Cannula Connectors
Cleaving Supplies
S90R	Fiber Optic Scribe	1	Alternate Cleavers
FS201	Fiber Inspection Scope	1	Alternate Inspection Tools
JEL10	Eye Loupe	1	Alternate Inspection Tools
BFG1	Bare Fiber Gripper	1	Fiber Termination Tools
Tools for Cannula Implantation
XCF	Stereotaxic Cannula Holder	1	Alternate Cannula Holders/ Adapters
XC7	Cannula Holder Adapter Arm	1
XC-CLAMP	Cannula Holder Adapter Clamp	1
Fiber Handling Supplies
TZ2	Optic Tweezers	1	Alternate Fiber Care Supplies
CKF	Fiber Cleaning Kit	1
FTDU	Fiber Disposal Unit	1

If using a fiber-coupled LED, the SM1L05 lens tube and SM1SMA adapter are not required. They may be removed from the default item list by changing the "Qty" box to 0 before clicking the "Add Kit" button.
We also offer our mating sleeves individually (Item # ADAF1).

Click to Enlarge

This kit features Ø200 μm fiber optic cannulae with Ø2.5 mm ferrules. This fiber size is less invasive for implantation, while the Ø2.5 mm ferrules provide easier handling and a more robust connection to the specimen.

Multiple patch cable options provide flexibility for a range of experimental setups. The standard patch cable is sufficient for sedated specimens. For experiments with mobile specimens, the rotary joint patch cable provides free movement of the cable via an integrated rotary joint interface. This reduces the risk of damage when the specimen moves, with higher light transmission and lower variation in rotational transmission than a separate rotary joint and patch cables solution.

To purchase this kit, its components can be added all at once to the shopping cart using the "Add Kit" button at the bottom of the ordering area, or individually using the shopping cart icon next to each item. Items may be removed from the default item list by changing the value in the "Qty" box to 0 before clicking the "Add Kit" button. To further customize the kit by adding alternative items, they can be added to the shopping cart via the alternate pages above or by using the "Quick View" links on the OG Selection Guide tab. This tab provides a comprehensive list of cannulae, sleeves, patch cables, and LEDs available for optogenetics studies.

Based on your currency / country selection, your order will ship from Newton, New Jersey

+1 Qty Docs Part Number - Universal Price Available

M470L5 470 nm, 809 mW (Min) Mounted LED, 1000 mA

$239.32