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Customizable Optogenetics Kits![]()
Kit with Rotary Joint Patch Cable, Ø200 µm Core Fiber, and Ø2.5 mm Ferrules Related Items ![]() Please Wait
Features
Thorlabs' optogenetics equipment is available in complete, ready-to-use kits. Our optogenetics kits include a 470 nm Fiber-Coupled LED with Each component in these kits can be removed or substituted for items more applicable to the individual needs of the experiment. See below for the most relevant kit, and the OG Selection Guide for For more particular experimental needs, Thorlabs offers custom fiber components for optogenetics. For more information, contact Tech Support. Optogenetics Product Family for In Vivo ApplicationsThorlabs offers a wide variety of products designed to support in vivo optogenetics applications. Please visit the OG Selection Guide tab above to see a full listing of available products for different applications. ![]()
Optogenetics Specifications
LED Specificationsa
Fiber Specifications
LED Driver Specifications
![]() Figure 1. Adjust the LEDD1B current limit adjuster arrow with a flathead screwdriver to set the maximum current (1.0 A or less). SetupLED and Driver Patch Cable and Cannula If you are using an ADAL1 (Ø1.25 mm ferrules) or ADAF1 (Ø2.5 mm ferrules) mating sleeve, place the mating sleeve onto the ferrule end of the patch cable. Leave approximately one-third of the mating sleeve length exposed for the cannula connection (Figure 2). Then, connect the mating sleeve to the cannula. To disconnect the cannula, grip the patch cable by the ferrule and mating sleeve and use a twisting motion. It is very important that the ends of the patch cable’s ferrule and the cannula’s ferrule are in physical contact (Figure 3). If they are not, the output power at the cannula tip will decrease significantly (Figure 4). ![]() Click to Enlarge Figure 2. The ADAF1 Mating Sleeve should be inserted 1/2 to 2/3 of the way onto the patch cable before cannula connection. ![]() Click to Enlarge Figure 3. Properly connected cannula and patch cable, with the cannula and patch cable in physical contact. ![]() Click to Enlarge Figure 4. Improperly connected cannula and patch cable, showing an air gap and light leakage. Quick-Release Interconnect Usage The ADAF2 (Ø2.5 mm ferrules) quick-release Rotary Joint Patch Cables OperationMake sure the current limit on the LED driver is set to 1.0 The LED can be operated using the LED driver Continuous Wave (CW) Mode Trigger (TRIG) Mode Modulation (MOD) Mode Fiber CarePatch cable connectors should be cleaned every time the cable is mated, tested, or reconfigured. Clean the fiber Further Reading
Fiber CleavingUncleaved cannula must be cleaved, or precision cut, before use. A high-quality fiber cleave will preserve the fidelity of the optical signal transmitted through the tip of the fiber. We recommend the following procedure for cleaving the fiber end. Required Materials
Starred (*) items are provided in kits with uncleaved cannulae. Cleaving Procedure
A good cleave will be flat across the fiber and perpendicular to the optic axis. There should be no ‘tag’ (i.e., protrusion) from the edge of the fiber. The region where the initial scribe was made may be visible. It should be less than 5% of the core diameter. Be patient as this process takes a little practice. Please be aware that it will be more difficult to achieve a high-quality cleave in large-core-diameter fibers compared to thinner fibers. A view of a properly cleaved fiber end as seen through a Thorlabs' JEL10 eye loupe is shown in Figure 4. Click Here for a Print Friendly Version of this Procedure Thorlabs also offers a full fiber termination manual, which covers fiber termination and bare fiber cleaving. It can be downloaded for free here, or a paper copy can be purchased here. †Kimwipes® is a registered trademark of the Kimberly-Clark Corporation.
Optogenetics Selection GuideThorlabs offers a wide range of optogenetics components; the compatibility of these products in select standard configurations is discussed in detail here. Please contact Technical Support for assistance with items outside the scope of this guide, including custom fiber components for optogenetics.
Single-Site StimulationOne Light Source to One Cannula ImplantThe most straightforward method for in vivo light stimulation of a specimen is to use a single fiber optic with a single LED light source. The single wavelength LED is powered by an LED driver, and then the illumination output is fiber-coupled into a patch cable, which connects to the implanted cannula. See the graphics and expandable compatibility tables below for the necessary patch cables and cannulae to create this setup. To choose the appropriate LED and driver, see below or the full web presentation. Click on Each Component for More Information ![]() Click to See Ø1.25 mm (LC) Ferrule Compatible Patch Cables, Cannulae, and InterconnectsClick to See Ø2.5 mm (FC) Ferrule Compatible Patch Cables, Cannulae, and Interconnects
Bilateral StimulationThe ability to accurately and simultaneously direct light to multiple locations within a specimen is desired for many types of optogenetics experiments. For example, bilateral stimulation techniques typically target neurons in two spatially separated regions in order to induce a desired behavior. In more complex experiments involving the simultaneous inhibition and stimulation of neurons, delivering light of two different monochromatic wavelengths within close proximity enables the user to perform these experiments without implanting multiple cannulae, which can increase stress on the specimen. Bilateral stimulation can be achieved with several different configurations depending on the application requirements. The sections below illustrate examples of different configurations using Thorlabs' optogenetics products. Option 1: One Light Source to Two Cannula Implants Using Rotary Joint SplitterThorlabs' RJ2 1x2 Rotary Joint Splitter is designed for optogenetics applications and is used to split light from a single input evenly between two outputs. The rotary joint interface allows connected patch cables to freely rotate, reducing the risk of fiber damage caused by a moving specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation. ![]() Click to See Ø1.25 mm (LC) Ferrule Components Recommended for Use with RJ2 Rotary Joint SplitterClick to See Ø2.5 mm (FC) Ferrule Components Recommended for Use with RJ2 Rotary Joint SplitterOption 2: One or Two Light Sources to Two Cannula ImplantsIf the intent is for one LED source to connect to two cannulae for simultaneous light modulation, then a bifurcated fiber bundle can be used to split the light from the LED into each respective cannula. For dual wavelength stimulation (mixing two wavelengths in a single cannula) or a more controlled split ratio between cannula, one can use a multimode coupler to connect one or two LEDs to the cannulae. If one cable end is left unused, the spare coupler cable end may be terminated by a light trap. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation. Click on Each Component Below for More Information ![]() ![]()
Two Light Sources into One Dual-Core Cannula ImplantFor bilateral stimulation applications where the two cannulas need to be placed in close proximity (within ~1 mm), Thorlabs offers dual-core patch cables and cannulae that are designed for this specific application. Each core is driven by a separate light source, enabling users to stimulate and/or supress nerve cells in the same region of the specimen. See the graphic and compatibility table below for the necessary cables and cannulae to create this setup. For LEDs and drivers, see below or the full web presentation. ![]() Click on Each Component for More Information
Illumination![]() Click to Enlarge M470F3 Fiber-Coupled LEDs and DriversOur fiber-coupled LEDs are ideal light sources for optogenetics applications. They feature a variety of wavelength choices and a convenient interconnection to optogenetics patch cables. Thorlabs offers fiber-coupled LEDs with nominal wavelengths ranging from 280 nm to 1050 nm. See the table to the right for the LEDs with the most popular wavelengths for optogenetics. A table of compatible LED drivers can be viewed by clicking below. ![]()
![]() Click to Enlarge This kit features Ø200 μm fiber optic cannulae with Ø2.5 mm ferrules. This fiber size is less invasive for implantation, while the Ø2.5 mm ferrules provide easier handling and a more robust connection to the specimen. Multiple patch cable options provide flexibility for a range of experimental setups. The standard patch cable is sufficient for sedated specimens. For experiments with mobile specimens, the rotary joint patch cable provides free movement of the cable via an integrated rotary joint interface. This reduces the risk of damage when the specimen moves, with higher light transmission and lower variation in rotational transmission than a separate rotary joint and patch cables solution. To purchase this kit, its components can be added all at once to the shopping cart using the "Add Kit" button at the bottom of the ordering area, or individually using the shopping cart icon next to each item. Items may be removed from the default item list by changing the value in the "Qty" box to 0 before clicking the "Add Kit" button. To further customize the kit by adding alternative items, they can be added to the shopping cart via the alternate pages above or by using the "Quick View" links on the OG Selection Guide tab. This tab provides a comprehensive list of cannulae, sleeves, patch cables, and LEDs available for optogenetics studies. ![]()
![]() Click to Enlarge This kit features Ø200 μm fiber optic cannulae with Ø1.25 mm ferrules, a standard patch cable, and a rotary joint patch cable. This fiber size is less invasive for implantation, while the Ø1.25 mm ferrules minimize stress on the specimen. Multiple patch cable options provide flexibility for a range of experimental setups. The standard patch cable is sufficient for sedated specimens. For experiments with mobile specimens, the rotary joint patch cable provides free movement of the cable via an integrated rotary joint interface. This reduces the risk of damage when the specimen moves, with higher light transmission and lower variation in rotational transmission than a separate rotary joint and patch cables solution. To purchase this kit, its components can be added all at once to the shopping cart using the "Add Kit" button at the bottom of the ordering area, or individually using the shopping cart icon next to each item. Items may be removed from the default item list by changing the value in the "Qty" box to 0 before clicking the "Add Kit" button. To further customize the kit by adding alternative items, they can be added to the shopping cart via the alternate pages above or by using the "Quick View" links on the OG Selection Guide tab. This tab provides a comprehensive list of cannulae, sleeves, patch cables, and LEDs available for optogenetics studies. ![]()
![]() Click to Enlarge This kit features Ø400 μm fiber optic cannulae with Ø2.5 mm ferrules and a standard patch cable. This fiber size is more durable for implantation with larger specimens, while the Ø2.5 mm ferrules provide easier handling and a more robust connection. This kit does not come with uncleaved cannulae or cleaving tools. Multiple patch cables options provide flexibility for a range of experimental setups. The standard patch cable is sufficient for sedated specimens. For experiments with mobile specimens, the rotary joint patch cable provides free movement of the cable via an integrated rotary joint interface. This reduces the risk of damage when the specimen moves, with higher light transmission and lower variation in rotational transmission than a separate rotary joint and patch cables solution. To purchase this kit, its components can be added all at once to the shopping cart using the "Add Kit" button at the bottom of the ordering area, or individually using the shopping cart icon next to each item. Items may be removed from the default item list by changing the value in the "Qty" box to 0 before clicking the "Add Kit" button. To further customize the kit by adding alternative items, they can be added to the shopping cart via the alternate pages above or by using the "Quick View" links on the OG Selection Guide tab. This tab provides a comprehensive list of cannulae, sleeves, patch cables, and LEDs available for optogenetics studies. ![]()
![]() Click to Enlarge This kit features Ø400 μm fiber optic cannulae with Ø1.25 mm ferrules and a standard patch cable. This fiber size is more durable for implantation with larger specimens, while the Ø1.25 mm ferrules minimize stress on the specimen. This kit does not come with uncleaved cannulae or cleaving tools. To purchase this kit, its components can be added all at once to the shopping cart using the "Add Kit" button at the bottom of the ordering area, or individually using the shopping cart icon next to each item. Items may be removed from the default item list by changing the value in the "Qty" box to 0 before clicking the "Add Kit" button. To further customize the kit by adding alternative items, they can be added to the shopping cart via the alternate pages above or by using the "Quick View" links on the OG Selection Guide tab. This tab provides a comprehensive list of cannulae, sleeves, patch cables, and LEDs available for optogenetics studies. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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