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Manual Patch-Clamp Micromanipulators


  • Manual Micromanipulator Assemblies with Precision Control
  • Ideal for Gibraltar™ Microscope Platforms
  • Position Pipette or Electrode Along Three Different Axes

Application Idea

Two PCS-5400 Micromanipulators Mounted on
Our Gibraltar GMHB-BX Platform

PCS-5300

Related Items


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 Common Micromanipulator Specifications
Control Type Fine (Piezo) Coarse
Travel 150 µm 300 µm 25 mm
Resolution <60 nm <120 nm 32 TPI
Speed Directly Proportional to Rate of Knob Turns N/A
Drift <1 µm/hr
Operator Interface Axis Control Unit Micrometer
Manipulator Dimensions
(L × W × H)
5.0" × 6.2" × 7.5"
(127 mm × 157 mm × 190.5 mm)

For questions and ordering details please contact Tech Support.

Features

  • Manual Micromanipulators with Piezoelectric Translation
  • Minimal Drift During Experiments: <1 µm/hr
  • Translation Along Three Axes:
    • 25 mm Coarse Travel (Micrometer)
    • 150 or 300 µm Fine Travel (Piezoelectric)
  • Repeatably Set Approach Angle and Rotation Using Adjustable Stop Rings
  • Headstage and Pipette Adapters Sold Separately
  • Include 60 V Power Supply

Thorlabs' Manual Micromanipulators offer excellent control of pipette manipulation for electrophysiology and life sciences research. These manual micromanipulators use piezoelectric (PZT) control of translation stages to provide smooth and precise movement of the pipette head. When combined with our Gibraltar Platforms, these micromanipulators achieve unparalleled stability and control. We also offer Motorized Patch-Clamp Micromanipulators with stepper motors and piezoelectric translation.

The micromanipulator can move along three different axes (shown in the image below). Coarse translation is controlled using a mechanical micrometer and produces a displacement of 25 mm at 32 TPI. Fine control on the micromanipulators is achieved with piezoelectric flexure mounts that provide a displacement of either 150 µm or 300 µm along each axis. Two adjustable stop rings enable the user to set an approach angle and rotational orientation for fast alignment during pipette swapping (see the Micromanipulator tab for more information). 

Adapters for positioning a headstage or pipette at steep or shallow approach angles can be purchased separately below. These adapters allow for more flexibility during experiments and minimize mechanical clashing with microscopy objectives or other experimental apparatus. A close-approach headstage and pipette adapter is also available that allows pipettes and headstages to be positioned closer to a sample without interference from the micromanipulator.

Piezoelectric Control
Piezoelectric control is used to achieve superior positioning compared to hydraulic manipulators, mechanical manipulators, or motorized lead screws. Piezoelectric control provides smooth and predictable movement with no backlash and minimal drift (<1 µm/hr with temperature control). An axis control unit is included with each piezoelectric micromanipulator (see the Control Unit tab). Each axis is controlled by an independent knob on the control unit and provides a displacement of 150 µm or 300 µm (depending on model). Three turns on the control unit corresponds to the full piezo travel range, resulting in a resolution of 0.04% of the total travel.

Power Supply
The PCS-5200, PCS-5300, and PCS-5400 assemblies include an axis control unit and 60 V power supply (see the Control Unit tab) which can support up to two micromanipulator assemblies. Since a single power supply can support two of our micromanipulators, assemblies without a power supply are available upon request (see the Custom Options tab). 
To order manual micromanipulators without a power supply, contact Tech Support.

PCS-5000 Micromanipulator Assembly
Click to Enlarge
Micromanipulator Assembly

Micromanipulator Assembly

Thorlabs’ Micromanipulator Assembly is composed of three linear translation stages, two rotary stages with lockable stops, one set of angle brackets, and a headstage mounting plate. The cross roller bearings of this assembly form a stiff and precise single-axis bearing system with high load capacity and minimal friction. The piezoelectric actuators provide 60 nm resolution, virtually no heating, zero backlash, and negligible drift (<1 µm/hr).

The headstage mounting plate easily accommodates headstages and pipettes. Note that the Axon CV-203B and HEKA EPC-9 headstages may be directly bolted to the headstage mounting plate. Other headstages may be mounted to the mounting plate by adding the appropriate mounting holes.

The headstage pivot allows for simple optimization of the pipette angle of approach on either an inverted or upright microscope. Additionally, the
PCS-500-SSH Steep/Shallow Headstage Adapter or MIS-PHM Pipette Holder may be used in applications requiring very steep or shallow angles. Due to the symmetric design of our micromanipulator assembly, this system can be easily modified for a left-sided or orthogonal approach axis without needing a special adapter kit.

Adjustable Stop Ring

PCS-5000 Mechanical Stop
Click to Enlarge
Adjustable Stop Ring with Pin and Brass Block with Locking Thumbscrew 

These micromanipulators include two adjustable stop rings that allow a user to repeatedly and accurately set the approach angle and horizontal rotational orientation (see image to the left). Once an appropriate angle or rotation is established, tighten the thumbscrew on the brass block to lock the mount in position. Loosen the two setscrews on either side of the pin using a 0.05" (1.3 mm) balldriver or hex key. Rotate the ring until the protruding pin is in physical contact with the brass block. To lock the ring, tighten the exposed setscrew, then loosen the thumbscrew to allow the mount to rotate freely. 

The adjustable stop rings in combination with the headstage adapter or pipette holder (sold separately below) provide a convenient mechanism for quickly changing pipettes and returning to the last position during an experiment, minimizing position readjustments and increasing efficiency. To do this, the user can set the adjustable stop ring to remember the approach angle and rotation, move the pipette away from the sample using the slide assembly on the adapters, rotate the stage away to exchange the pipette, and then quickly return to same approach angle and rotation as before.

PCS-5000 Control Cube
Click to Enlarge
Axis Control Unit

Axis Control Unit

The axis control unit allows the user to adjust pipette placement by hand with smooth and repeatable movement. Each of the three stages that comprise our micromanipulator assembly can connect to one of the potentiometer knobs on the axis control unit. For convenience during system configuration, the user may choose any knob to control any particular stage. This allows the user to set the axis control unit in the most intuitive configuration for the system.

Each knob controls a three-turn potentiometer, which regulates voltage to the piezo flexure assembly and thus its position. Three turns correspond to the full travel range of the piezo assembly, yielding a resolution that is 0.04% of the total piezo travel range. For example, a 150 µm piezo stage will have a 60 nm resolution provided by the axis control unit. Additionally, the user may set the potentiometer friction to suit experimental needs.

PCS-5000 Power Supply
Click to Enlarge
60 V Power Supply

Power Supply

Our micromanipulator power supply provides a regulated, low noise, 60 V output for the axis control unit. Two voltage outputs enable the power supply to regulate two axis control units. This allows the user to run two micromanipulation assemblies from the same power source, saving money and space (see the Custom Options tab for more information). This power supply accepts an input power range of 100 – 240 V at 50/60 Hz.

Custom Options for Manual Micromanipulators 

In addition to the manual micromanipulators available from stock, micromanipulator assemblies without a power supply are available as custom options. The PCS-520N assembly has no PZT actuators or motors and cannot be used with a power supply. The remaining custom options are identical to the micromanipulators we offer from stock; however they do not include a power supply.

photo detector power supply
Click to Enlarge

The PCS-5200N Connected to Included Axis Control Unit
Item #a Power
Supply
Coarse Travelb Fine Travelc Description
All Axes Y Axis Z Axis Approach Axis
PCS-520N No 25 mm - - - Mechanical Version; No PZT Actuators or Motors;
Cannot Be Used with a Power Supply
PCS-5200N No 25 mm 150 µm 150 µm 150 µm 150 µm PZT Travel in All Axes
PCS-5300N No 25 mm 150 µm 150 µm 300 µm Recommended for working in slices where longer
PZT travel on the approach axis is required.
PCS-5400N No 25 mm 300 µm 300 µm 300 µm Recommended for working in thick slices
where maximum flexibility is needed.
  • Click the Item # for a Photo
  • Mechanical Control Using Micrometer
  • Piezoelectric Control Using Axis Control Unit

For questions and ordering details, please contact Tech Support.


Posted Comments:
aabizet  (posted 2018-04-10 06:03:19.14)
We need a REPLACEMENT PART for PIEZO MOTOR of MICROMANIPULATOR PSC 6000-300.
YLohia  (posted 2018-04-10 09:27:46.0)
Hello, thank you for contacting Thorlabs. We will reach out to you directly to find out what needs to be replaced/repaired.
bikashp  (posted 2013-12-16 14:50:24.48)
I used a PCS-5000 for four years bought for $5,800.00. Now after four years the cost of repair for the manipulator is $5,682.00. So the actual cost of this manipulator is $17,000.00 for 12 years of usage.
jlow  (posted 2014-01-16 04:43:43.0)
Response from Jeremy at Thorlabs: I have spoken with our Burleigh group and they informed that a resolution was reached with a revised repair quote of around $3700. A similar micromanipulator would cost around $8750 new. After looking at the serial number of the unit, it was determined that your unit is around 7 years old.
k.ozer  (posted 2013-07-26 08:26:17.33)
Dear Sir/Madam, We have bought a PCS-6000 from Burleigh about ~3 years ago and now want to do a modification on one of its axes. Can a technical support engineer contact me please. Thanks in advance. Best regards, Kerem Ozer Spektralab Ltd. +90 312 447 7709
jlow  (posted 2013-08-01 08:51:00.0)
Response from Jeremy at Thorlabs: Thank you for contacting Thorlabs. Someone from our Technical Support group will contact you on this.

Micromanipulators with Power Supply

These micromanipulator assemblies include a 60 V power supply. A single power supply can support two micromanipulators. To order manual micromanipulators without a power supply, please visit the Custom Options tab above.

Item # Power
Supply
Coarse Travela
Fine Travelb Description
All Axes Y Axis Z Axis Approach Axis
PCS-5200 Yes 25 mm 150 µm 150 µm 150 µm 150 µm PZT Travel in All Axes
PCS-5300 Yes 25 mm 150 µm 150 µm 300 µm Recommended for working in slices where longer
PZT travel on the approach axis is required.
PCS-5400 Yes 25 mm 300 µm 300 µm 300 µm Recommended for working in thick slices
where maximum flexibility is needed.
  • Mechanical Control Using Micrometer
  • Piezoelectric Control Using Axis Control Unit
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
PCS-5200 Support Documentation
PCS-5200Micromanipulator Assembly, 150 µm Travel for X, Y, and Approach
$8,434.16
Today
PCS-5300 Support Documentation
PCS-5300Micromanipulator Assembly, 150 µm Travel for X & Y, 300 µm Travel for Approach
$8,964.61
Today
PCS-5400 Support Documentation
PCS-5400Micromanipulator Assembly, 300 µm Travel for X, Y, and Approach
$9,813.33
Today

Micromanipulator Headstage Adapter and Pipette Holder Mounts


Click to Enlarge
The PCS-500-SSH can be mounted for steep (left) or shallow (right) approach angles.
  • Mount Headstages or Pipettes onto Manual or Motorized Micromanipulator Assemblies
  • Three Options Available:
    • PCS-500-SSH: Two Dovetails with a Mounting Platform for Large Axon or HEKA Headstages
    • PCS-AXN-ADP: Single-Dovetail, Close-Approach Adapter for Smaller Headstages
    • MIS-PHM: Two Dovetails with Pipette Mount that Provides 360° Rotational Positioning
  • Smooth Motion Allows Orientation of Headstage or Pipette at Very Steep (>45°) or Very Shallow (<25°) Angles
  • Multiple Mounting Position Options Provide Extra Clearance Near Objectives

These adapters for the micromanipulator assemblies provide a flexible platform for mounting a headstage or pipette; see the table below for details. Each adapter alows the mounted headstage or pipette to be positioned at steep or shallow approach angles. Steep approach angles are often desired in electrophysiology experiments in order to minimize the length of the electrode in solution, ensuring that electrical noise is kept to a minimum. In contrast, slice electrophysiology experiments often use electrodes positioned nearly parallel (15° to 25° angle) to the experimental surface to allow the pipette tip to be moved in a straight line over the sample chamber. Shallow approach angles also enable users to stack multiple pipettes or headstages in close proximity. In both these cases, extra clearance for steep or shallow approach angles is needed to prevent interference with the microscope or other experimental apparatus.


Click to Enlarge
PCS-500-SSH Headstage Adapter Components

Steep/Shallow Headstage Adapter
The PCS-500-SSH Steep/Shallow Angle Headstage Adapter features a flexible mounting platform for Axon CV-5, Axon CV-203B, and HEKA EPC-9 headstages. The adapter consists of the headstage adapter plate, the slide assembly with a dovetail on the back, a clamping plate, and a mounting plate (shown in the image to the right). Using the adapter, users can quickly exchange pipettes during an experiment.

The mounting plate provides two possible dovetail grooves for securing the slide. For shallow approach angles, the slide is placed within the lower dovetail bracket, while for steep approach angles, the slide is placed in the upper dovetail bracket (see the image above to the right). Mounting in this manner provides maximum clearance above the micromanipulator when it is rotated for a steep angle approach.

To install the adapter, attach the mounting plate to the micromanipulator using the included 4-40 screws (3/32" hex). Secure the slide assembly in one of the dovetail grooves by installing the clamping plate with the included 2-56 cap screws and 5/64" (2 mm) hex key. The user can loosen the clamping plate and position the entire slide assembly; this determines the stop locations that correspond to the fully extended and fully retracted positions of the pipette. At least 1" of the slide assembly should be held by the clamping plate to ensure stability of the mount. To adjust the slide position, turn the locking handle to loosen, then move the slide to the desired location, and retighten the locking handle.


Click for Details
The PCS-AXN-ADP Close-Approach Adapter Secured to a Micromanipulator

Click to Enlarge
MIS-PHM Pipette Holder Mount Secured to Micromanipulator

Close Approach Headstage Adapter
The PCS-AXN-ADP Headstage Adapter is a compact solution for the close approach mounting of a smaller headstage or pipette mount (sold separately). This compact solution provides more clearance room for other equipment.

It is attached to the micromanipulator using the headstage adapter plate included with the micromanipulator and the included 2-56 cap screws and 5/64" (2 mm) hex key. When mounted to this adapter, the PCS-AXN-ADP will be offset from the micromanipulator body, allowing it to be used in closer proximity to the experiment while reducing mechanical clashing. Please see the manual for detailed mounting instructions.

A single dovetail groove is provided to hold a headstage or pipette mount. The groove is the same width as the other headstage adapters, making it compatible with the headstage adapter plate included with the PCS-500-SSH and the pipette mount included with the MIS-PHM.

Pipette Holder Mount
The MIS-PHM Pipette Holder Mount can hold pipettes up to Ø4 mm and position them at steep or shallow approach angles. It uses the same mounting plate and dovetail clamping mechanism as the PCS-500-SSH Headstage Adapter, which allows for positioning of the slide position and two mounting locations. The pipette clamp can freely rotate 360°, allowing for coarse control of the pipette rotation angle. Fine control of the approach angle is accomplished using the micromanipulator itself.

For users who have an older pipette holder, please contact Tech Support for information on replacement options.

Item # Compatibility Approach Anglesb Mounting
Headstage Pipette Mounta
PCS-500-SSH Axon CV-203B
Axon CV-5
HEKA EPC-9
Yes
(Not Included)
Smooth Motion for
Steep (>45°) or
Shallow (<25°)
Mounts Directly to Micromanipulator
PCS-AXN-ADP Axon CV-203B
Axon CV-7B
Yes
(Not Included)
Close Approach, Requires Headstage Adapter Plate
(Included with Micromanipulators)
MIS-PHM Yes
(Included)
Mounts Directly to Micromanipulator
  • A pipette mount is included with the MIS-PHM. It is compatible, but not included, with the PCS-AXN-ADP and PCS-500-SSH.
  • Steep and shallow approach angles can be achieved using the PCS-500-SSH and MIS-PHM by using the top or bottom dovetail on the back plate.
Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
PCS-500-SSH Support Documentation
PCS-500-SSHSteep/Shallow Headstage Adapter
$827.82
Today
PCS-AXN-ADP Support Documentation
PCS-AXN-ADPClose Approach Headstage Adapter
$220.75
Today
MIS-PHM Support Documentation
MIS-PHMPipette Holder Mount
$851.62
Today
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