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Precision Cover Glasses and Microscope Slides


  • #1.5H (170 µm) and #0 (85 - 115 µm) Thickness Cover Glasses
  • 26.0 mm x 76.0 mm Glass Microscope Slides
  • 25.4 mm x 76.2 mm Fluorescent Microscope Slides
  • Ideal for High-Performance Imaging Applications

MS10PC

Positively Charged Flat Slide

CG15KH

Rectangular Cover Glass

MS15C1

Slide with Single Cavity

CG15XH

Ø25 mm Cover Glass

Application Idea

Slides Mounted in a Microscope using MLS203-1 XY Stage and MLS203P10 Multiple Slide Holder Stage Accessory

FSK1

Blue Fluorescent Slide

FSK2

Green Fluorescent Slide

FSK6

Red Fluorescent Slide

Related Items


Please Wait
Item # Thickness Description Dimensions Quantity
Cover Glasses
CG15CH #1.5H
(170 ± 5 µm)
Square 22 mm x 22 mm 1000
CG15CH2 200
CG15KH Rectangle 24 mm x 50 mm 1000
CG15KH1 100
CG15NH Small Circle Ø12 mm 1000
CG15NH1 100
CG15XH Large Circle Ø25 mm 1000
CG15XH1 100
CG00C #0
(85 - 115 µm)
Square 22 mm x 22 mm 1000
CG00C2 200
CG00K Rectangle 24 mm x 50 mm 1000
CG00K1 100
Microscope Slides
MS10UW 1 mm Flat 26 mm x 76 mm 1000
MS10UW2 200
MS10PC Flat,
Positively Charged
1000
MS10PC1 100
MS15C1 1.35 mm Single Cavity 50
MS15C2 Dual Cavity 50
Fluorescent Microscope Slides
FSK1(-P5) 1.7 mm Fluorescent, Blue 25.4 mm x 76.2 mm 1 or 5
FSK2(-P5) Fluorescent, Green 1 or 5
FSK3(-P5) Fluorescent, Yellow 1 or 5
FSK4(-P5) Fluorescent, Orange 1 or 5
FSK6(-P5) Fluorescent, Red 1 or 5
FSK5 Fluorescent Set, All Colors 5

Features

  • Designed for High-Performance Microscopy Applications
  • Cover Slips Made from Schott D 263® M Glass
    • Precision, #1.5H (170 ± 5 µm) Thickness: Square, Rectangular, and Round Versions Available
    • #0 (85 - 115 µm) Thickness: Square and Rectangular Versions Available
  • 26 mm x 76 mm (W x L) Slides Made from Soda-Lime Glass
    • 1 mm Thick Slides with Positively Charged or Untreated Surfaces
    • 1.35 mm Thick Slides with Cavity Wells for Samples
  • 25.4 mm x 76.2 mm Fluorescent Slides Made from a Cast Acrylic Substrate
    • 1.7 mm Thick Fluorescent Slides
    • Available in Five Colors: Blue, Green, Yellow, Orange, and Red
    • Each Slide Sold Individually or in Packs of Five
    • FSK5 Slide Set Includes All Five Colored Slides

Thorlabs offers a selection of Precision Cover Glasses (Cover Slips), Microscope Slides, and Fluorescent Slides (see the table to the right) designed for high-performance imaging applications such as total internal reflection fluorescence (TIRF) microscopy, live cell imaging, and biochemistry.

Precision Cover Glasses
Cover glasses are manufactured with Schott D 263® M Glass, which is optically clear, colorless, and exhibits low auto-fluorescence. #1.5H cover slips are machined precisely to maintain a uniform thickness of 170 ± 5 µm, which minimizes image defects due to spherical aberration; we offer square, rectangular, and round versions. #0 cover slips are ground to a thickness of 85 - 115 µm, providing the thinnest cover slip thickness for weakly fluorescent or thick samples; square and rectangular versions are available.

Microscope Slides
The 26 mm x 76 mm glass slides are manufactured from corrosion-resistant, soda-lime glass. 1 mm thick slides feature a white marking region and are available with an untreated surface or positively charged surface for added adhesion. 1.35 mm thick slides feature the choice of one or two cavities and have untreated surfaces. Slides can be incorporated into slide holders that are compatible with 26 mm x 76 mm slides, like our microscopy slide and test target holders. Thorlabs' XY and XYZ high-speed motorized stages for microscopy can be fitted with inserts for holding slides in a variety of configurations.

Fluorescent Microscope Slides
The Fluorescent Microscope Slides are available in five colors: blue, green, yellow, orange, and red. Fabricated from cast acrylic substrates, they fluoresce at wavelengths that are similar to many popular fluorophores when illuminated with LEDs or lasers commonly used in widefield, confocal, or multiphoton microscopy. Each slide provides a uniform fluorescent field across its surface area, useful for aligning fluorescence imaging systems. All slides are compatible with our sample holders that fit 26 mm x 76 mm, 1.7 ± 0.5 mm thick slides. Please note that our C4SH01 Four-Position Microscope Slide Holder is not compatible with these slides.

Cover Glass Specifications
Type Schott D 263® M
Dimensions 22 mm x 22 mm,
24 mm x 50 mm,
Ø12 mm, or Ø25 mm
22 mm x 22 mm or
24 mm x 50 mm
Thickness #1.5H (170 ± 5 µm) #0 (85 - 115 µm)
Average Transmissiona,b 91.7 ± 0.1%
Refractive Index at 589.3 nm 1.523
Coefficient of Thermal Expansion 7.2 x 10-6 K-1
Abbe Number 55
Density 2.51 g/cm3
  • Specified using the τvD65 test for a 0.15 mm thick sample.
  • Sample transmission data can be viewed in the Graphs tab.

Microscope Slide Glass Specifications
Surface Flat,
Untreated
Flat,
Positively Charged
Cavity Wells,
Untreated
Type Soda Lime
Dimensions 26 mm x 76 mm
Thickness 1 mm ± 0.05 mm 1 mm ± 0.05 mm 1.35 mm ± 0.15 mm
Average Transmissiona,b 91.2 ± 1%
Refractive Index at 589.3 nm 1.523
Coefficient of Thermal Expansion (20 - 300 °C) 9 x 10-6 K-1
Density 2.5 g/cm3
Elastic Modulus 7.3 x 104 N/mm2
Poisson Ratio 0.23
Alkaline Resistance (ISO 695) Class 1 - 2 Class 2 Class 1 - 2
Acid Resistance (ISO 8424) Class 1 Class 3 Class 1
Hydrolytic Resistance (ISO 719) Class 3 Class 3 Class 3
  • Specified using the τvD65 test for a 1 mm thick sample.
  • Sample transmission data can be viewed in the Graphs tab.

Fluorescent Slide Specificationsa
Type Cast Acrylic
Dimensions 25.4 mm x 76.2 mm
Thickness 1.7 mm ± 0.2 mm
  • Sample transmission data can be viewed in the Graphs tab.

Microscope Slides and Cover Glasses


Click to Enlarge
Transmission measurements of four CG00C cover glasses from 350 nm to 800 nm. These data represent all #0 thickness cover glasses.

Click to Enlarge
Transmission measurements of four CG15CH cover glasses from 350 nm to 800 nm. These data represent all #1.5H thickness cover glasses.

Click to Enlarge
Transmission measurements of four MS10UW microscope slides from 350 nm to 800 nm. These data represent all microscope slides.

Fluorescent Slides


Click to Enlarge
Raw Data
Comparison of transmission measurements of the FSK1 (blue), FSK2 (green), FSK3 (yellow), FSK4 (orange), and FSK6 (red) fluorescent slides from 300 nm to 700 nm. This data is typical; performance may vary from lot to lot.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK1 blue fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. This data is typical; performance may vary from lot to lot.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK2 green fluorescent slide when illuminated with the M470F3 470 nm Fiber-Coupled LED. This data is typical; performance may vary from lot to lot.

Click to Enlarge

Raw Data
Measured fluorescence spectrum of the FSK3 yellow fluorescent slide when illuminated with the M365FP1 365 nm Fiber-Coupled LED. This data is typical; performance may vary from lot to lot.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK4 orange fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. This data is typical; performance may vary from lot to lot.

Click to Enlarge
Raw Data
Measured fluorescence spectrum of the FSK6 red fluorescent slide when illuminated with the M565F3 565 nm Fiber-Coupled LED. This data is typical; performance may vary from lot to lot.

There are a several common techniques to prepare a sample for microscopy with a slide and cover glass. 

Dry Mount
In this method, the sample is placed directly on the slide. To protect the microscope objective, a cover glass should be placed over the sample. For large samples, the MS15C1 or MS15C2 slides with wells may be used for additional space.

Wet Mount Preparation

Click to Enlarge
Use a tweezer to lower the cover slip on top of the sample.

Click to Enlarge
Use a dropper bottle to place a drop of fluid on the slide.

Wet Mount
To prepare a wet mount, place the sample on the slide. Then, use a pipette or B2939 dropper bottle to add a drop of liquid (e.g., water, glycerin, growth media, etc.) on top of the specimen. Using a pair of tweezers, hold the cover glass on one end and carefully lower it over the sample droplet (see the images to the right). 

  • Ensure that there are no air bubbles trapped underneath the cover slip.
  • Too much liquid underneath the cover glass will cause it to slide. Use a small piece of paper towel or KW32 Kimwipes®† near the edge of the cover slip to absorb excess fluid.
  • If there is too little liquid underneath the cover glass, use a pipette or dropper bottle to add a small drop of fluid near the edge of the cover slip.

To protect the mounted sample from evaporation or contamination, the cover glass can be sealed or glued to the microscope slide. For live cells or other sensitive samples seal the edges with valap, which is an equal parts mixture of Vaseline, lanolin, and paraffin wax. When slowly melted together, the mixture can be applied to the edges of the cover glass with a small brush or cotton swab.

Smear Preparation
For this type of sample mounting, first place a drop of the sample to be smeared on the slide. Using the edge of a second slide, gently smear the sample to create a thin, uniform layer. Place a cover slip over the smear and use a paper towel or KW32 Kimwipes to absorb any excess fluid from the edge of the cover slip.

Please note: Do not use ethanol with the Fluorescent Calibration Microscope Slides because it may cause damage to the slides and bleed through.

Kimwipes® is a registered trademark of the Kimberly-Clark Corporation.


Posted Comments:
yannick.folwill  (posted 2018-10-25 15:42:24.663)
Dear Thorlabs, for me the diameter of the cover glasses is very critical, I was using the 25 mm substrates and built a mount for it which worked fine for the first two batches I got. Now the new batch of substrates I have is of diameter ~25.2 mm which is too large for my mount. Do you also have those glasses with tighter tolerances or at least leaning to have smaller diameter? Thank you.
YLohia  (posted 2018-10-31 11:19:36.0)
Hello, thank you for contacting Thorlabs. My apologies for the issues caused by the larger size of your latest cover glass order. We have a tolerance of +/-0.5mm on these. I will reach out to you directly to discuss the possibility of offering a tighter tolerance on these.
nevgilhooly88  (posted 2018-09-07 15:49:58.673)
We recently tried CG15CH2 coverslips as a replacement for GoldSeal coverslips from Fisher. Both coverslips are made with D263M glass. However, the CG15CH2 coverslips showed horrendous autofluorescence straight out the box compared to theGoldSeals. I had to bake the coverslips at 450oC to remove the fluorescence. Are these coverslips pure D263M glass or has it been doped with other material? I'm concerned that our surface chemistries will not work as well with these coverslips.
llamb  (posted 2018-09-21 09:00:26.0)
Thank you for your feedback. The CG15CH2 cover slips are made from pure D 263 M Schott glass. The raw glass material itself is gas coated with sulfur dioxide, which can connect on the glass surface with sodium oxide to become sodium sulfate. The intensity/amount of this coating may vary. This process is done by the raw glass manufacturer to ensure that the glass sheets can be easily separated. Perhaps your coverslips are autofluorescing from a particularly thick coating, or there may be additional impurities in the glass that are causing this. I will reach out to you directly to troubleshoot further and see if replacement coverslips are necessary.
ysung4  (posted 2018-08-07 15:28:38.073)
Can you make coverslips that can be used with your sample holder (https://www.thorlabs.com/thorproduct.cfm?partnumber=MPSH)?
YLohia  (posted 2018-08-16 08:25:23.0)
Hello, thank you for your feedback. I have posted this new product idea for 35mm diameter coverslips on our internal engineering forum for further consideration.
jbiegen  (posted 2017-03-26 13:44:45.313)
Can you tell me what the diameter tolerance is on the CG15XH 25mm cover slip? Also, can you broadband AR coat one of the surfaces and if so, what is best surface quality could you provide? Thanks.
tfrisch  (posted 2017-04-18 09:53:27.0)
Hello, thank you for contacting Thorlabs. While we don't provide dimensional tolerances for these cover slips, we may be able to provide a custom window for your application. I will reach out to you directly about your requirements.
pzhao  (posted 2016-08-22 09:28:14.193)
CG15CH is made of Schott D263. Compared with Fused Silica, does it have higher or lower autofluorescence? Thanks.

Precision Cover Glasses, #1.5H (170 µm) Thickness


Click to Enlarge
24 mm x 50 mm CG15KH Cover Slip Wet Mounted to MS10UW Slide
  • Precision Glass Cover Slips with #1.5H (170 µm) Thickness and ± 5 µm Tolerance
  • Recommended for Use with High NA Objective Lenses
  • Square (22 mm x 22 mm), Rectangular (24 mm x 50 mm), and Round (Ø12 mm and Ø25 mm) Versions

Thorlabs' Precision Cover Glasses are ground to a uniform thickness of 170 ± 5 µm and are made from Schott D 263 M Glass, an optically clear and colorless glass that exhibits low auto-fluorescence. The uniform thickness and consistent optical properties help reduce image defects due to spherical aberrations (see the Specs tab for details). As such, these cover slips are ideal for use with high NA objective lenses.

Cover glasses are available in square (22 mm x 22 mm), rectangular (24 mm x 50 mm), and round (Ø12 mm and Ø25 mm) sizes. Please note that the Ø12 mm cover slips cannot be used with the MS15C1 and MS15C2 cavity slides. For additional information on mounting dry or wet samples with cover glasses, please see the Slide Preparation tab.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
CG15CH Support Documentation
CG15CHCustomer Inspired! Precision Cover Glasses, #1.5H Thickness, 22 x 22 mm, Pack of 1000
$128.77
Today
CG15CH2 Support Documentation
CG15CH2Customer Inspired! Precision Cover Glasses, #1.5H Thickness, 22 x 22 mm, Pack of 200
$30.24
Today
CG15KH Support Documentation
CG15KHCustomer Inspired! Precision Cover Glasses, #1.5H Thickness, 24 x 50 mm, Pack of 1000
$304.08
Today
CG15KH1 Support Documentation
CG15KH1Customer Inspired! Precision Cover Glasses, #1.5H Thickness, 24 x 50 mm, Pack of 100
$36.07
Today
CG15NH Support Documentation
CG15NHCustomer Inspired! Precision Cover Glasses, #1.5H Thickness, Ø12 mm, Pack of 1000
$308.40
Today
CG15NH1 Support Documentation
CG15NH1Customer Inspired! Precision Cover Glasses, #1.5H Thickness, Ø12 mm, Pack of 100
$40.31
Today
CG15XH Support Documentation
CG15XHCustomer Inspired! Precision Cover Glasses, #1.5H Thickness, Ø25 mm, Pack of 1000
$489.12
Today
CG15XH1 Support Documentation
CG15XH1Customer Inspired! Precision Cover Glasses, #1.5H Thickness, Ø25 mm, Pack of 100
$62.59
Today

Cover Glasses, #0 (85 - 115 µm) Thickness

  • Glass Cover Slips with #0 (85 - 115 µm) Thickness
  • Square (22 mm x 22 mm) and Rectangular (24 mm x 50 mm) Versions

Thorlabs' #0 Cover Glasses are ground to a thickness of 85 - 115 µm and are made from Schott D 263 M Glass, an optically clear and colorless glass that exhibits low auto-fluorescence. These cover slips are the thinnest standard thickness, covering the sample with the least optic material. This is ideal for weakly fluorescent or thick samples.

Cover glasses are available in square (22 mm x 22 mm) and rectangular (24 mm x 50 mm) sizes. For additional information on mounting dry or wet samples with cover glasses, please see the Slide Preparation tab.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
CG00C Support Documentation
CG00CCustomer Inspired! Cover Glasses, #0 Thickness, 22 x 22 mm, Pack of 1000
$103.97
Today
CG00C2 Support Documentation
CG00C2Customer Inspired! Cover Glasses, #0 Thickness, 22 x 22 mm, Pack of 200
$29.71
Today
CG00K Support Documentation
CG00KCustomer Inspired! Cover Glasses, #0 Thickness, 24 x 50 mm, Pack of 1000
$263.10
Today
CG00K1 Support Documentation
CG00K1Customer Inspired! Cover Glasses, #0 Thickness, 24 x 50 mm, Pack of 100
$51.98
Today

Microscope Slides, 26 mm x 76 mm

  • 26 mm x 76 mm (W x L) Soda-Lime Glass Slides
  • Corrosion Resistant (Hydrolytic Class 3 Rating); See the Specs Tab for Details
  • Pre-Cleaned and Ready for Use; Autoclavable 
  • 1 mm Thick Flat Slides with White Marking Region
    • Untreated Surface, Packs of 1000 or 200
    • Positively Charged Surface, Packs of 1000 or 100
  • 1.35 mm Thick Slides with One or Two Cavity Wells, Packs of 50

These 26 mm x 76 mm (W x L) Microscope Glass Slides are manufactured using a corrosion-resistant, soda-lime glass with a hydrolytic class 3 rating for compatibility with most sample types and imaging applications. The slides are pre-cleaned and ready for use directly from storage; for further cleaning, they can be autoclaved prior to use. Glass slides are available in 1 mm or 1.35 mm thick versions. The 1 mm thick flat glass slides are offered with a 26 mm x 20 mm white marking region on one side for easy labeling; use a pencil for best results. The slide surface is offered untreated, or positively charged for additional sample adhesion. The 1.35 mm thick slides contain one or two Ø15 - Ø18 mm, 0.6 - 0.8 m deep spherical cavities for working with liquid samples, tissue cultures, or larger specimens.

Slides can be mounted in slide holders such as the MAX3SLH Fixed Microscope Slide Holder or into slide holder inserts for motorized and manual microscopy stages. These slides are compatible with all slide holders that fit 26 mm x 76 mm slides.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
MS10UW Support Documentation
MS10UWCustomer Inspired! Microscope Slides, 1 mm Thick, White Marking Region, Pack of 1000
$146.08
Today
MS10UW2 Support Documentation
MS10UW2Customer Inspired! Microscope Slides, 1 mm Thick, White Marking Region, Pack of 200
$41.38
Today
MS10PC Support Documentation
MS10PCCustomer Inspired! Positively Charged Microscope Slides, 1 mm Thick, White Marking Region, Pack of 1000
$579.25
Today
MS10PC1 Support Documentation
MS10PC1Customer Inspired! Positively Charged Microscope Slides, 1 mm Thick, White Marking Region, Pack of 100
$72.14
Today
MS15C1 Support Documentation
MS15C1Customer Inspired! Microscope Slides, 1.35 mm Thick, Single Cavity, Pack of 50
$155.83
Today
MS15C2 Support Documentation
MS15C2Customer Inspired! Microscope Slides, 1.35 mm Thick, Two Cavities, Pack of 50
$242.39
Today

Fluorescent Microscope Slides, 25.4 mm x 76.2 mm


Click to Enlarge

Raw Data
Fluorescence of the five slides when illuminated with Thorlabs' Fiber-Coupled LEDs. Please see the raw data file for the LEDs used to obtain these fluorescence spectra. This data is typical; performance may vary from lot to lot. Please see the Graphs tab for more details.

Click to Enlarge
The FSK6 Red Fluorescent Slide Mounted Using the MP100-RCH2 Rigid Stand Slide Holder
  • Available in Five Colors: Blue, Green, Yellow, Orange, and Red
    • Each Slide Sold Individually or in Packs of Five
    • FSK5 Slide Set Includes All Five Colored Slides
  • 25.4 mm x 76.2 mm and 1.7 mm Thick
  • Five Different Fluorescence Spectra Similar to a Variety of Fluorophores (See Graph to the Right)
  • Provides a Uniform Fluorescent Field Across the Surface Area
  • Made of a Cast Acrylic Substrate

Our Fluorescent Microscope Slides are designed to be used to align fluorescence imaging systems. Commonly used with widefield, confocal, and multiphoton imaging systems, these slides provide a uniform fluorescent field across their surface area and have fluorescence spectra similar to a variety of fluorophores. Each slide is 25.4 mm x 76.2 mm and is cut from cast acrylic material with a thickness of 1.7 ± 0.5 mm. A plastic storage box is included with the FSK5 Slide Set and the packs of five slides to keep the them clean and dust-free. The box is engraved with the Thorlabs logo and item # on the top. For custom sizes up to 1' x 2', please contact Tech Support. For more details on these fluorescent slides, please see the full web presentation.

These slides are compatible with our sample holders that fit 26 mm x 76 mm, 1.7 ± 0.5 mm thick slides. Please note that our C4SH01 Four-Position Microscope Slide Holder is not compatible with these slides.

Based on your currency / country selection, your order will ship from Newton, New Jersey  
+1 Qty Docs Part Number - Universal Price Available
FSK1 Support Documentation
FSK1Fluorescent Microscope Slide, Blue, 1.7 mm Thick
$5.15
Today
FSK1-P5 Support Documentation
FSK1-P5Fluorescent Microscope Slide, Blue, 1.7 mm Thick, 5 Pack
$18.54
Today
FSK2 Support Documentation
FSK2Fluorescent Microscope Slide, Green, 1.7 mm Thick
$5.15
Today
FSK2-P5 Support Documentation
FSK2-P5Fluorescent Microscope Slide, Green, 1.7 mm Thick, 5 Pack
$18.54
Today
FSK3 Support Documentation
FSK3Fluorescent Microscope Slide, Yellow, 1.7 mm Thick
$5.15
Today
FSK3-P5 Support Documentation
FSK3-P5Fluorescent Microscope Slide, Yellow, 1.7 mm Thick, 5 Pack
$18.54
Today
FSK4 Support Documentation
FSK4Fluorescent Microscope Slide, Orange, 1.7 mm Thick
$5.15
Today
FSK4-P5 Support Documentation
FSK4-P5Fluorescent Microscope Slide, Orange, 1.7 mm Thick, 5 Pack
$18.54
Today
FSK6 Support Documentation
FSK6Fluorescent Microscope Slide, Red, 1.7 mm Thick
$5.15
Today
FSK6-P5 Support Documentation
FSK6-P5Fluorescent Microscope Slide, Red, 1.7 mm Thick, 5 Pack
$18.54
Today
FSK5 Support Documentation
FSK5Fluorescent Microscope Slides, Set of 5 Colors
$19.10
Today
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