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Low-Autofluorescence Multimode Patch Cables![]()
MAF1L1 Patch Cable with FC/PC Connectors MAF2L1 Patch Cable with FC/PC Connector and Ø1.25 mm Ferrule MAF3L1 Patch Cable with FC/PC Connector and Ø2.5 mm Ferrule Related Items ![]() Please Wait
Please note that these patch cables cannot be sterilized using an autoclave. Users can alternatively apply a light mist of an aqueous mixture containing Virkon™ disinfectant.
![]() Click to Enlarge Click Here for Raw Data Plot comparing the recovery of autofluorescence for a low-autofluorescence (AF) and standard patch cable after photobleaching at 470 nm for 12 hours. An M470F3 LED was used for excitation and autofluorescence intensity at 525 nm was measured relative to the output power from the patch cable. Features
These multimode patch cables are manufactured with components that reduce the emitted autofluorescence in the visible spectrum. This makes these patch cables suitable for fiber photometry applications where high sensitivity is required to measure the changes in fluorescence that indicate neural activity within a specimen. The design of these patch cables is based on testing of the autofluorescent properties of our patch cable components such as the bare fiber, ferrule types, and epoxies. Each patch cable incorporates a Ø400 µm, 0.50 NA multimode fiber (Item # FP400URT) and is available with three connector configurations. One end is equipped with an FC/PC connector, while the other is equipped with an FC/PC connector, Ø1.25 mm stainless steel ferrule, or a Ø2.5 mm stainless steel ferrule. Similar to our standard optogenetics patch cables, the patch cables with a ferrule end can be mated to a fiber optic cannula using an interconnect or mating sleeve; see the selection guide below for compatible products. Each patch cable includes two protective caps that shield the ferrule ends from dust and other hazards when not in use. Additional plastic, metal, or threaded caps for the connector and ferrule ends are sold separately here. If the fiber ends become dirty from use, we offer a selection of inspection tools, as well as fiber optic cleaning products. Photobleaching In our product testing, we compared the autofluorescence recovery of a low-autofluorescent patch cable to a standard patch cable. The graph to the right shows the measured autofluorescence relative to the output power for each test patch cable over a period of 36 days. We observe that the low-autofluorescent patch cable has a lower autofluorescence ratio immediately after photobleaching and also exhibits a slower recovery of autofluorescence over the test period. Mating Sleeve and Patch Cable Compatibility *Low autofluorescence was tested and verified for excitation at 470 nm and 565 nm. Performance may vary for excitation at other wavelengths.
![]() Click to Enlarge Photo showing a setup for photobleaching a low-autofluorescence patch cable.
Example Photobleaching ProcedureFiber photometry experiments measure fluorescence emitted from genetically-modified cells as an indicator of neural activity. To obtain the sensitivity needed for resolving the small changes in fluorescence emissions, noise from other sources, such as the optical patch cables, must be minimized. The most common fluorophore used in fiber photometry is green fluorescent protein (GFP) which is excited at wavelengths near 470 nm. Photobleaching is a process where the fluorophores in a sample or material are saturated with light at the excitation wavelength. This makes it a useful technique for reducing the emitted autofluorescence in a patch cable prior to a fiber photometry experiment. We recommend that users photobleach their patch cables before each experiment. The steps below detail a general procedure that can be used.
Test Setup for Autofluorescence MeasurementIn our performance testing, Thorlabs measured the emitted autofluorescence from these patch cables at an excitation wavelength of 470 nm. The experimental setup used is shown in the diagram below; click on the link below the diagram for a list of parts for excitation at 470 nm and measuring autofluorescence at 525 nm. ![]() Click to Enlarge Schematic of Autofluorescence Measurement Setup for 470 nm Excitation In this setup, a DFM1 dichroic filter cube is fitted with fiber collimators and imaging filters to direct light to and from the low-autofluorescence patch cable. Excitation light from the fiber-coupled LED is directed by the dichroic cube to the low-autofluorescence patch cable, which is left uncapped and open to air. Autofluorescent emissions at 525 nm in the patch cable are coupled through the fiber and dichroic filter to the S130C detector. Using this setup, we measured the autofluorescence relative to the output power during the photobleaching process (graph below and to the left) and as the autofluorescence recovers after photobleaching (graph below and to the right). We observe that overnight exposure (>8 hours) greatly reduces the autofluorescence of the patch cable and that the recovery of a low-autofluorescence patch cable is slower than a standard patch cable. ![]() Click to Enlarge Click Here for Raw Data Plot comparing the recovery of autofluorescence for a low-autofluorescence (AF) and standard patch cable after photobleaching at 470 nm for 12 hours. Autofluorescence intensity at 525 nm was measured relative to the output power from the patch cable. The test setup for measurement is shown to the right. ![]() Click to Enlarge Click Here for Raw Data Plot of autofluorescence ratio (measured at 525 nm) versus photobleaching time at 470 nm. Autofluorescence intensity at 525 nm was measured relative to the output power from the patch cable. The test setup for photobleaching and measurement is shown to the right.
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