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Products Home / Optical Systems / Objective Lenses, Scan Lenses, and Tube Lenses / Super Apochromatic Microscope ObjectivesSuper Apochromatic Microscope Objectives
- Infinity-Corrected Dry Microscope Objectives
- Super Apochromatic Axial Color Correction
- 1X, 2X, 4X, or 10X Magnification
TL10X-2P
10X Super Apochromat Objective
for Two-Photon Microscopy
Application Idea
Mount four of our super apochromatic objectives on a CSN500 objective turret with a PLE351 parfocal length extender for the TL4X-SAP objective and an M32A2 thread adapter for the TL10X-2P objective.
TL2X-SAP
2X Super Apochromat Objective
TL1X-SAP
1X Super Apochromat Objective
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| Objective Lens Selection Guide |
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| Objectives |
| Super Apochromatic Microscope Objectives Microscopy Objectives, Dry Microscopy Objectives, Oil Immersion Physiology Objectives, Water Dipping or Immersion Phase Contrast Objectives Long Working Distance Objectives Reflective Microscopy Objectives UV Microscopy Objectives VIS and NIR Focusing Objectives |
| Scan Lenses and Tube Lenses |
| Scan Lenses Infinity-Corrected Tube Lens |
Did You Know?
Multiple optical elements, including the microscope objective, tube lens, and eyepieces, together define the magnification of a system. See the Magnification & FOV tab to learn more.
| Webpage Features | |
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Zemax blackbox files for the objectives on this page can be accessed by clicking this icon below. |
Features
- Infinity-Corrected Super Apochromatic Design
- Antireflection (AR) Coatings:
- 1X Objective: 420 nm - 700 nm
- 2X and 4X Objectives: 350 nm - 700 nm
- 10X Objective: 400 nm - 1300 nm
- Numerical Aperture (NA) up to 0.5
- Ideal for Brightfield, Fluorescence, Multiphoton, and Confocal Imaging Applications
- Magnifications Specified for Use with a 200 mm Tube Lens
Thorlabs' super apochromatic microscope objectives provide axial color correction in the visible range and a flat field of focus in a variety of imaging modalities without introducing vignetting. These objectives are designed for use with a tube lens focal length of 200 mm and have optical elements that are AR-coated for improved transmission. Our 1X telecentric objective is ideal for machine vision applications (see Telecentric Tutorial tab), while our 2X and 4X objectives have high numerical apertures (NA), making them ideal for widefield imaging. Lastly, our 10X objective is designed for two-photon imaging. This objective has a long 7.77 mm working distance and features a correction collar and optical thickness scale, allowing the objective focus to be adjusted for a variety of media that may be present between the objective and the sample, such as aqueous solutions and cover glasses (see the Correction Collar tab for details). Note that these objectives are not suitable for water-dipping, water-immersion, or oil-immersion techniques.
Every objective housing is engraved with the Item #, magnification, NA, wavelength range, and working distance. Each objective is shipped in an objective case comprised of a lid and container. The threading for each objective is given below; to use the objectives with a different thread standard, please see our microscope objective thread adapters.
Mounting the objectives on this page together or in combination with other objectives may require parfocal length extenders, which allow users to match parfocal lengths and switch between objectives of different magnifications mounted in a turret or nosepiece without significant refocusing.
| Item # | TL1X-SAPa | TL2X-SAP | TL4X-SAP | TL10X-2P |
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| Specifications | ||||
| Magnificationb | 1X | 2X | 4X | 10X |
| Numerical Aperture (NA) | 0.03 | 0.10 | 0.20 | 0.5 |
| AR Coating Range | 420 - 700 nm | 350 - 700 nm | 400 - 1300 nm | |
| AR Coating Reflectance Per Surface |
Ravg < 0.5% @ 0° AOI | Rabs < 2.5% (400 - 450 nm) Rabs < 1.75% (450 - 1300 nm) @ 0° - 25° AOI |
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| Total Transmisison (Click to Enlarge) |
 Raw Data |
 Raw Data |
 Raw Data |
 Raw Data |
| Axial Color | Diffraction Limited over 440 - 700 nmc | Diffraction Limited over |
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| Field of View | Ø22 mm | Ø11 mm | Ø5.5 mm | Ø2.2 mm |
| Working Distanced | 8.0 mm | 56.3 mm | 17.0 mm | 7.77 mm |
| Effective Focal Length (EFL) | 200 mm | 100 mm | 50 mm | 20 mm |
| Entrance Pupil Diameter | 12 mme | 20 mme | 20 mme | 20 mmf |
| Parfocal Lengthd | 95.0 mm | 95.0 mm | 60.0 mmg | 95.0 mm |
| Diameterd | 32.6 mm (Without Wave Plate) |
30.5 mm | 43.2 mm | |
| 34.5 mm (With Wave Plate) |
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| Lengthd | 85.5 mm (Without Wave Plate) |
43.5 mm | 46.4 mm | 90.4 mm |
| 90.6 mm (With Wave Plate) |
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| Housing Threads | M25 x 0.75 | M32 x 0.75 | ||
| Thread Depth | 3.8 mm | 3.2 mm | 3.6 mm | 3.2 mm |
| Field Numberh | 22 | |||
| Design Tube Lens Focal Lengthi | 200 mm | |||
| Cover Glass Thickness | 0 - 5.0 mm | 0 - 5.0 mm | 0 - 5.0 mm | 0 - 2.6 mm |
| Recommended Microscopy Techniques | ||||
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1X Objective Performance Graphs
Click here for raw data for all plots.
Click to Enlarge
Transmission of the TL1X-SAP Objective. The blue shaded region denotes the wavelength range of the AR coating.
Click to Enlarge
Axial color describes the shift in the image plane across the operating wavelength range of the 1X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance.
Click to Enlarge
The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL1X-SAP over its field of view is shown in the graph above.
Click to Enlarge
This graph shows the Strehl Ratio of the TL1X-SAP over the objective's full operating wavelength range.
2X Objective Performance Graphs
Click here for raw data for all plots.
Click to Enlarge
Transmission of the TL2X-SAP Objective. The blue shaded region denotes the wavelength range of the AR coating.
Click to Enlarge
Axial color describes the shift in the image plane across the operating wavelength range of the 2X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance.
Click to Enlarge
The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL2X-SAP over its field of view is shown in the graph above.
Click to Enlarge
This graph shows the Strehl Ratio of the TL2X-SAP over the objective's full operating wavelength range.
4X Objective Performance Graphs
Click here for raw data for all plots.
Click to Enlarge
Transmission of the TL4X-SAP Objective. The blue shaded region denotes the wavelength range of the AR coating.
Click to Enlarge
Axial color describes the shift in the image plane across the operating wavelength range of the 4X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance.
Click to Enlarge
The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL4X-SAP over its field of view is shown in the graph above.
Click to Enlarge
This graph shows the Strehl Ratio of the TL4X-SAP over the objective's full operating wavelength range.
10X Objective Performance Graphs
Click here for raw data for all plots.
Click to Enlarge
Transmission of the TL10X-2P Objective. The blue shaded region denotes the wavelength range of the AR coating.
Click to Enlarge
Axial color describes the shift in the image plane across the operating wavelength range of the 10X super apochromatic microscope objective. The pink shaded region denotes diffraction-limited performance. Diffraction-limited performance can be shifted to the NIR with refocusing.
Click to Enlarge
The Strehl Ratio is a quantitative measurement of optical image formation quality. The Strehl Ratio for the TL10X-SAP over its field of view is shown in the graph above.
Click to Enlarge
This graph shows the Strehl Ratio of the TL10X-2P over the objective's visible performance band.
Click for Details
Above shows a diagram of a TL4X-SAP; Thorlabs maintains the labeling convention shown above for all of its super apochromatic microscope objectives.
Glossary of Terms
Magnification
The magnification of an objective is the lens tube focal length (L) divided by the objective's focal length (F):
M = L / F .
The total magnification of the system is the magnification of the objective multiplied by the magnification of the eyepiece or camera tube. The specified magnification on the microscope objective housing is accurate as long as the objective is used with a compatible tube lens focal length.
Numerical Aperture (NA)
Numerical aperture, a measure of the acceptance angle of an objective, is a dimensionless quantity. It is commonly expressed as
NA = ni × sinθa
where θa is the maximum 1/2 acceptance angle of the objective, and ni is the index of refraction of the immersion medium. This medium is typically air, but may also be water, oil, or other substances.
Parfocal Length
Also referred to as the parfocal distance, this is the length from the top of the objective (at the base of the mounting thread) to the bottom of the cover glass (or top of the specimen in the case of objectives that are intended to be used without a cover glass). For instances in which the parfocal length needs to be increased, parfocal length extenders are available.
Working Distance
This is the distance between the front element of the objective and the specimen, depending on the design of the objective. The cover glass thickness specification engraved on the objective designates whether a cover glass should be used.
Field Number
The field number corresponds to the size of the field of view (in millimeters) multiplied by the objective's magnification.
FN = Field of View Diameter × Magnification
Correction Collar
Some objectives are equipped with a correction collar to compensate for differences in cover glass thickness or other media present between the objective and the plane of interest within the sample. The collar is used to adjust the relative position of an internal focusing element or group of elements. See the Correction Collar tab for more information.
Click to Enlarge
This graph shows the effect of a cover slip on image quality at 632.8 nm for a #1.5 cover glass (0.17 mm thickness) with a refractive index of 1.51.
Click for Details
Thickness and Light Cone Dramatized for Readability
Correction Collar and Cover Glass Correction
A cover glass is often placed onto an aqueous specimen to create a flat top on a volume to image. While this makes focusing on a sample easier, the presence of the glass can introduce spherical aberrations in the final image. The graph to the right shows the magnitude of spherical aberration versus the thickness of the cover glass used, for 632.8 nm light. For the typical cover glass thickness of 0.17 mm, the spherical aberration caused by the cover glass does not exceed the diffraction-limited aberration for objectives with NA up to 0.40.
Our TL10X-2P microscope objective is equipped with a correction collar, which compensates for cover glasses of different thickness by adjusting the relative position of internal optical elements, reducing the impact of spherical aberration to help achieve diffraction-limited performance. Its correction collar features two scales: one for the thickness of a cover glass with a refractive index of 1.51 and a second that corresponds to the optical thickness of the material between the objective and the focal plane (ignoring air). The latter is useful for cases where the region of interest is suspended in a solution. Values on both scales are given in units of millimeters.
To find the optical thickness of all non-air elements between your objective and your sample, multiply the thickness of an element in millimeters by its refractive index, and find the sum of all such products. For example, suppose that the TL10X-2P objective is focusing through 7.30 mm of air, a 0.17 mm cover slip, and 1.00 mm of saline solution, as illustrated in the image to the lower right. To find the appropriate correction collar setting, multiply each thickness, t, by each material's refractive index, n, ignoring air:
Optical Thickness Collar Setting = ∑txnx
t1n1 + t2n2 = 0.17 mm × 1.51 + 1.00 mm × 1.33 = 1.59 mm
| Examples of Optical Thickness Calculationsa | ||
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| Glass Thickness (n = 1.51) |
Water Thickness (n = 1.33) |
Optical Thickness Collar Setting |
| 0.00 mm | 0.00 mm | 0.00 mm |
| 0.50 mm | 0.00 mm | 0.76 mm |
| 0.17 mm | 1.00 mm | 1.59 mm |
| 0.17 mm | 3.00 mm | 4.25 mm |
Our other super apochromatic objectives do not have a correction collar. While they are designed for use with a standard 0.17 mm thick cover glass, their NAs are low enough (0.2 or less) that any cover glass thickness between 0 and 0.5 mm can be used with little impact on image quality.
Telecentric Lenses
Telecentric lenses are designed to have a constant magnification regardless of the object's distance or location in the field of view. This attribute is ideal for many machine vision measurement applications, as measurements of an object's dimension will be independent of where it is located.
Types of Telecentric Lenses
In order to achieve a telecentric lens design, all of the chief rays (rays from an off-axis point that pass through the center of the aperture stop) have to be parallel to the optical axis in either image space or object space, or both. For an object space telecentric lens, the chief rays will be parallel to the optic axis on the object's side of the lens (object space). This is accomplished by setting the aperture stop at the front focal plane of the lens, which results in an entrance pupil at infinity. Since chief rays are directed towards the center of the entrance pupil, the chief rays on the object side of the lens will be parallel to the optical axis. An example of an object space telecentric lens is shown in Figure 1.
Figure 1: A ray trace through an idealized object space telecentric lens system. Note that the chief rays (the center ray of each color) is parallel to the optical axis in object space, but in image space, the chief rays form an angle with the optical axis.
For an image space telecentric lens, the chief rays will be parallel to the optical axis on the image's side of the lens (image space). This is accomplished by setting the aperture stop at the back focal plane of the lens, which results in an exit pupil at infinity. Since the chief rays must pass through the center of the exit pupil, the chief rays on the image side of the lens must be parallel to the optical axis. For a double telecentric, or bi-telecentric, lens, the front and back focal planes are made to overlap so that the aperture stop is located where both the entrance and exit pupils are at infinity. In a bi-telecentric lens, neither the image or object location will affect the magnification. Figure 2 shows an example ray trace through a telecentric lens and illustrates how the chief rays pass through the system.
Figure 2: A ray trace through an idealized bi-telecentric lens system. Note that the chief rays (the center ray of each color) is parallel to the optical axis in both image and object space, which means that the magnification will remain constant regardless of object distance.
Conventional Lenses
In conventional lenses, the entrance and exit pupils are not located at infinity, so the chief rays will not be parallel to the optical axis. In this case, the magnification of the object depends on its distance from the lens and its position in the field of view. Figure 2 shows an example ray trace through a conventional camera lens; notice how the chief rays are angled with respect to the optical axis in both image and object space. Note that both Figures 2 and 3 feature the same lens design; only the aperture stop location was varied to shift the telecentric system to a non-telecentric one.
Figure 3: A ray trace through an idealized conventional camera lens system. Note that the chief rays (the center ray of each color) is not parallel to the optic axis in both image and object space, which means that the magnification will vary with object distance.
Example Images
Figures 4 and 5 show photographs taken with a telecentric lens and a standard camera lens, respectively. With the telecentric imaging system, the height of the two screws appears to be the same, even though the object planes are separated by approximately 45 mm along the optical axis. With the conventional imaging system, the two screws appear to be different heights, and therefore a machine vision system based on this lens will lead to incorrect dimensional measurements.
Click to Enlarge
Figure 4: An image of two identical 8-32 cap screws imaged with our previous generation MVTC23013 0.128X Telecentric Lens. Although the screws appear to be the same size and in the same object plane, they are actually separated by a distance of approximately 45 mm along the optical axis.
Click to Enlarge
Figure 5: The same two screws photographed with our MVL7000 conventional zoom lens. In this image, the perspective error due to the separation of the screws would lead to incorrect height measurements.
When viewing an image with a camera, the system magnification is the product of the objective and camera tube magnifications. When viewing an image with trinoculars, the system magnification is the product of the objective and eyepiece magnifications.
| Manufacturer | Tube Lens Focal Length |
|---|---|
| Leica | f = 200 mm |
| Mitutoyo | f = 200 mm |
| Nikon | f = 200 mm |
| Olympus | f = 180 mm |
| Thorlabs | f = 200 mm |
| Zeiss | f = 165 mm |
Magnification and Sample Area Calculations
Magnification
The magnification of a system is the multiplicative product of the magnification of each optical element in the system. Optical elements that produce magnification include objectives, camera tubes, and trinocular eyepieces, as shown in the drawing to the right. It is important to note that the magnification quoted in these products' specifications is usually only valid when all optical elements are made by the same manufacturer. If this is not the case, then the magnification of the system can still be calculated, but an effective objective magnification should be calculated first, as described below.
To adapt the examples shown here to your own microscope, please use our Magnification and FOV Calculator, which is available for download by clicking on the red button above. Note the calculator is an Excel spreadsheet that uses macros. In order to use the calculator, macros must be enabled. To enable macros, click the "Enable Content" button in the yellow message bar upon opening the file.
Example 1: Camera Magnification
When imaging a sample with a camera, the image is magnified by the objective and the camera tube. If using a 20X Nikon objective and a 0.75X Nikon camera tube, then the image at the camera has 20X × 0.75X = 15X magnification.
Example 2: Trinocular Magnification
When imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. If using a 20X Nikon objective and Nikon trinoculars with 10X eyepieces, then the image at the eyepieces has 20X × 10X = 200X magnification. Note that the image at the eyepieces does not pass through the camera tube, as shown by the drawing to the right.
Using an Objective with a Microscope from a Different Manufacturer
Magnification is not a fundamental value: it is a derived value, calculated by assuming a specific tube lens focal length. Each microscope manufacturer has adopted a different focal length for their tube lens, as shown by the table to the right. Hence, when combining optical elements from different manufacturers, it is necessary to calculate an effective magnification for the objective, which is then used to calculate the magnification of the system.
The effective magnification of an objective is given by Equation 1:
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(Eq. 1) |
Here, the Design Magnification is the magnification printed on the objective, fTube Lens in Microscope is the focal length of the tube lens in the microscope you are using, and fDesign Tube Lens of Objective is the tube lens focal length that the objective manufacturer used to calculate the Design Magnification. These focal lengths are given by the table to the right.
Note that Leica, Mitutoyo, Nikon, and Thorlabs use the same tube lens focal length; if combining elements from any of these manufacturers, no conversion is needed. Once the effective objective magnification is calculated, the magnification of the system can be calculated as before.
Example 3: Trinocular Magnification (Different Manufacturers)
When imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. This example will use a 20X Olympus objective and Nikon trinoculars with 10X eyepieces.
Following Equation 1 and the table to the right, we calculate the effective magnification of an Olympus objective in a Nikon microscope:
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The effective magnification of the Olympus objective is 22.2X and the trinoculars have 10X eyepieces, so the image at the eyepieces has 22.2X × 10X = 222X magnification.
Sample Area When Imaged on a Camera
When imaging a sample with a camera, the dimensions of the sample area are determined by the dimensions of the camera sensor and the system magnification, as shown by Equation 2.
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(Eq. 2) |
The camera sensor dimensions can be obtained from the manufacturer, while the system magnification is the multiplicative product of the objective magnification and the camera tube magnification (see Example 1). If needed, the objective magnification can be adjusted as shown in Example 3.
As the magnification increases, the resolution improves, but the field of view also decreases. The dependence of the field of view on magnification is shown in the schematic to the right.
Example 4: Sample Area
The dimensions of the camera sensor in Thorlabs' 1501M-USB Scientific Camera are 8.98 mm × 6.71 mm. If this camera is used with the Nikon objective and trinoculars from Example 1, which have a system magnification of 15X, then the image area is:
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Sample Area Examples
The images of a mouse kidney below were all acquired using the same objective and the same camera. However, the camera tubes used were different. Read from left to right, they demonstrate that decreasing the camera tube magnification enlarges the field of view at the expense of the size of the details in the image.
Click to Enlarge
Old Objective Packaging
Click to Enlarge
New Objective Packaging
Smart Pack Goals
- Reduce Weight of Packaging
- Increase Usage of Recyclable Materials
- Improve Packing Integrity
- Decrease Shipping Costs
Thorlabs' Smart Pack Initiative is aimed at minimizing waste while providing adequate protection for our products. By eliminating any unnecessary packaging, implementing design changes, and utilizing eco-friendly materials, this initiative seeks to reduce the environmental impact of our product packaging.
The new super achromatic microscope objective packaging is made from over 60% recycled paper products and weighs 25.44 g less than the old packaging. The transition from foam and non-recycled cardboard to recycled paper products results in a 68.05% reduction in the amount of CO2 produced per kg of packing materials. All products on this page have transitioned, or are in the process of transitioning to recycled paper.
As we move through our product line, we will indicate re-engineered, eco-friendly packaging with our Smart Pack logo, which can be seen in the image to the right.
| Posted Comments: | |
Xindong Song
(posted 2021-02-25 16:31:18.73) Hi Thorlabs, we are very interested in getting the TL10X-2P objective for in vivo 2p imaging. But before we place the order, we wonder if we can talk to any other customers about their experience with the objective. Thank you. Michael Orger
(posted 2020-11-06 06:51:53.37) Hi, Can you define Forward and Reverse for the TL10X-2P zemax black box files? It seems to be the opposite of what I expect, but I am not sure if it is a misunderstanding on my part. Thanks! asundararaj
(posted 2020-11-10 09:40:01.0) Thank you for contacting Thorlabs. Forward is typically defined as sending a collimated light into the objective, and the reverse is defined as sample being on the object side. Forward is always from collimation to focus. Gabriel Martins
(posted 2019-07-17 06:54:01.707) good morning,
do you have information on how the 10x performs with cleared samples, ie, samples in medium with refractive index of ~1.52?
Would it be possible to test the objective with our samples?
Thanks in advance,
Gaby asundararaj
(posted 2019-07-23 10:08:15.0) Thank you for contacting Thorlabs. Yes, the TL10X-2P objective can be used with cleared samples. You would need to set the spherical collar to the appropriate setting. You can find a procedure for calculating the correct setting in the Correction Collar tab of this page. I have contacted you directly about being able to offer a loan unit. Denis Pristinski
(posted 2019-07-12 12:53:38.397) Is Zemax black box model coming for TL10X-2P? YLohia
(posted 2019-07-16 09:02:38.0) Hello, thank you for contacting Thorlabs. The Zemax model for this can be found here : https://www.thorlabs.com/_sd.cfm?fileName=TTN142896-S02.zar&partNumber=TL10X-2P a.scagliola2
(posted 2018-11-05 12:38:56.0) Do you have a model of the objective with the principal planes? nbayconich
(posted 2019-03-04 01:17:21.0) Thank you for contacting Thorlabs. The principal plane will be located approximately 16mm in front of the objective lens or 16mm after the m25 x0.75 threading. I will reach out to you directly with more information. a.andreski
(posted 2017-11-28 15:40:24.44) Do these objectives have a flat field of view (are they plan)?
If I use the TL2X-SAP with a TTL100A tubelens, what is the maximum object field of view that is possible without abberations?
Thanks. tfrisch
(posted 2017-12-15 04:35:36.0) Hello, thank you for contacting Thorlabs. The Field of View will be flat, yes. TL2X-SAP will be diffraction limited over its full Field of View. I will reach out to you directly with details on the Strehl ratio. ludoangot
(posted 2016-11-07 15:31:29.6) Which tube lens do you recommend to match the extended transmission of these microscope lenses in the deep blue / UV (your recent TTL200 is for 400 to 700nm)? tfrisch
(posted 2016-11-10 10:24:31.0) Hello, thank you for contacting Thorlabs. I would typically recommend TTL200 which performs well over the range of TL2X-SAP and TL4X-SAP. You can see some performance graphs here: https://www.thorlabs.us/newgrouppage9.cfm?objectgroup_id=5834&tabname=Graphs |
Zoom
Click to Enlarge
Click Here for Full-Size Image
10 kΩ resistor and diode surface mounted to a circuit board, imaged using the TL1X-SAP objective with reflected illumination from a FRI61F50 Ring Light and a color camera. The objective's telecentric design provides constant magnification through the depth of the image.
- Ideal for Machine Vision Applications
- Telecentric Design with Wave Plate for Reducing Back Reflections
- M25 x 0.75 Threading
Click to Enlarge
The TL1X-SAP objective includes a removable wave plate that is attached to end of the objective barrel with magnets.
The TL1X-SAP Super Apochromatic Objective provides diffraction-limited axial color performance over 440 nm to 700 nm. The telecentric design provides constant magnification regardless of the object's distance or location in the field of view; see the Telecentric Tutorial tab for more information. This objective has a Ø22 mm field of view and is ideal for machine vision applications. It includes a removable waveplate that minimizes back reflections when used with an epi-illuminated system, thus enabling an increase in contrast. Magnets in the objective housing secure the wave plate in place. White markings on the 1X objective barrel and a black dot on the wave plate provide a reference when rotating the wave plate.
The full-size download of the circuit board image to the right should be viewed using ThorCam, ImageJ, or other scientific imaging software. It may not be displayed correctly in general-purpose image viewers.
| Key Specifications | ||||||||||||||
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| Item # | AR Coating Wavelength Range |
Mag. | Numerical Aperture |
Working Distance |
Parfocal Length |
Cover Glass Thickness |
Threading | Recommended Microscopy Techniques | ||||||
| Confocal | Two-Photon | Brightfield | Darkfield | Dodt | DIC | Fluorescence | ||||||||
| TL1X-SAP | 420 - 700 nm | 1X | 0.03 | 8.0 mma | 95.0 mma | 0 - 5.0 mm | M25 x 0.75 | |
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Zoom- High Numerical Aperture for Low-Light and Widefield Imaging
- M25 x 0.75 Threading
These general-purpose objectives provide diffraction-limited axial color performance over 400 nm to 750 nm. They offer high-quality performance when used with a variety of imaging modalities and are useful for viewing large regions of a sample. The TL2X-SAP objective features an Ø11 mm field of view, large enough to capture the full length of an embryonic Zebrafish (Figure 1 below). The 4X magnification of the TLX4X-SAP can resolve finer details than its 2X counterpart, while the Ø5.5 mm field of view still provides a sense of scope and place within the sample (Figures 2 and 3 below). The long working distance of each objective provides ample room for micromanipulators.
The full-size downloads of the three images below should be viewed using ThorCam, ImageJ, or other scientific imaging software. They may not be displayed correctly in general-purpose image viewers.
Click to EnlargeClick Here for Full-Size Image
Figure 1: Embryonic zebrafish embryo imaged using the TL2X-SAP objective, white light illumination, and a color camera. The image captures the full length of the zebrafish while still retaining fine details.
Click to EnlargeClick Here for Full-Size Image
Figure 2: Dicot flower bud imaged using the TL4X-SAP objective and monochrome camera in a DIC setup.
Click to EnlargeClick Here for Full-Size Image
Figure 3: Confocal fluorescence image of a mouse retina labeled with GFP, taken using the TL4X-SAP objective.
| Key Specifications | ||||||||||||||
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| Item # | AR Coating Wavelength Range |
Mag. | Numerical Aperture |
Working Distance |
Parfocal Length |
Cover Glass Thickness |
Threading | Recommended Microscopy Techniques | ||||||
| Confocal | Two-Photon | Brightfield | Darkfield | Dodt | DIC | Fluorescence | ||||||||
| TL2X-SAP | 350 - 700 nm | 2X | 0.10 | 56.3 mm | 95.0 mm | 0 - 5.0 mm | M25 x 0.75 | |
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| TL4X-SAP | 350 - 700 nm | 4X | 0.20 | 17.0 mm | 60.0 mm | 0 - 5.0 mm | M25 x 0.75 | |
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Zoom- Dry Objective Ideal for Two-Photon Microscopy
- Correction Collar Enables Use with Samples in a Variety of Media
- M32 x 0.75 Threading
The TL10X-2P 10X Super Apochromatic Objective combines the diffraction limited axial color performance at visible wavelengths of our other objects with excellent transmission out to 1300 nm, making this objective ideal for multiphoton imaging applications (see the Graphs tab for details). It combines a high 0.50 NA for collecting two-photon fluorescence signals with a long 7.77 mm working distance, providing ample space for equipment to manipulate the sample. A correction collar allows adjustment for spherical aberrations introduced by imaging through aqueous solutions or thick cover glasses, without the need for water dipping or oil immersion (see the Correction Collar tab).
Note that for best performance, the front focal plane of your system's tube lens should be placed at the TL10X-2P's aperture stop. For most objectives, this stop coincides with the back element at the shoulder. This objective's aperture stop is located 42 mm from its shoulder. Without filling this stop, vingetting may occur. For example, Thorlabs' TTL200MP tube lens' focal plane is located at 228 mm from the shoulder of the tube lens. Therefore, the physical separation between the front element of the tube lens and the shoulder of the TL10X-2P objective should be 184 mm. This example is illustrated in the diagram to the lower left.
Due to the large outer diameter of this objective's housing, it may not mate properly with nosepieces that have recessed M32 x 0.75 internal threads such as Item #s CSN200 and CSN210. In this case, the M32M32S brass microscope adapter has external and internal M32 x 0.75 threads that can provide additional clearance between the edge of the objective housing and the mount.
The full-size download of the mouse brain image to the lower right should be viewed using ThorCam, ImageJ, or other scientific imaging software. It may not be displayed correctly in general-purpose image viewers.
Click to EnlargeClick Here for Full-Size Image
Wild Type Mouse Brain Section (30 µm) Tagged with DAPI (405 nm) and Alexa 488 Anti-Neurofilament Taken with TL10X-2P Objective (Courtesy of Lynne Holtzclaw of the National Institutes of Health)
Click to Enlarge
This diagram shows how to position the TL10X-2P relative to the TTL200MP tube lens so the entrance pupil is completely filled.
| Key Specifications | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Item # | AR Coating Wavelength Range |
Mag. | Numerical Aperture |
Working Distance |
Parfocal Length |
Cover Glass Thickness |
Threading | Recommended Microscopy Techniques | ||||||
| Confocal | Two-Photon | Brightfield | Darkfield | Dodt | DIC | Fluorescence | ||||||||
| TL10X-2P | 400 - 1300 nm | 10X | 0.50 | 7.77 mm | 95.0 mm | 0 - 2.6 mm | M32 x 0.75 | |
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